Study on alleviating effect of Glycyrrhizae Radix et Rhizoma on Psoraleae Fructus-induced liver injury based on network pharmacology and cell experiments.
10.19540/j.cnki.cjcmm.20211025.401
- Author:
Ya-Li WU
1
;
Xiao-Yan WANG
2
;
Hui ZHANG
1
;
Yu-Long CHEN
3
;
Ming-Liang ZHANG
1
;
Xiao-Fei CHEN
1
;
Lu NIU
1
;
Wei-Xia LI
4
;
Jin-Fa TANG
4
Author Information
1. Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China.
2. Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China Provincial and Ministerial Co-construction Collaborative Innovation Center for Prevention and Treatment of Respiratory Diseases with Traditional Chinese Medicine, Henan University of Chinese Medicine Zhengzhou 450046, China.
3. Henan University of Chinese Medicine Zhengzhou 450046, China.
4. Henan Province Engineering Laboratory for Clinical Evaluation Technology of Chinese Medicine, Henan Provincial Key Laboratory for Clinical Pharmacy of Traditional Chinese Medicine, the First Affiliated Hospital of Henan University of Chinese Medicine Zhengzhou 450000, China Henan University of Chinese Medicine Zhengzhou 450046, China Provincial and Ministerial Co-construction Collaborative Innovation Center for Prevention and Treatment of Respiratory Diseases with Traditional Chinese Medicine, Henan University of Chinese Medicine Zhengzhou 450046, China.
- Publication Type:Journal Article
- Keywords:
Glycyrrhizae Radix et Rhizome;
Psoraleae Fructus;
liver injury;
network pharmacology;
pharmacodynamic effects
- MeSH:
Chemical and Drug Induced Liver Injury, Chronic;
Drugs, Chinese Herbal/pharmacology*;
Glycyrrhiza;
Humans;
Medicine, Chinese Traditional;
Network Pharmacology
- From:
China Journal of Chinese Materia Medica
2022;47(1):176-187
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to explore the alleviating effect and mechanism of Glycyrrhizae Radix et Rhizoma against Psora-leae Fructus-induced liver injury based on network pharmacology and cell experiments. The active components of Glycyrrhizae Radix et Rhizoma and Psoraleae Fructus were first retrieved from the Encyclopedia of Traditional Chinese Medicine(ETCM), Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP), Comparative Toxicogenomics Database(CTD), and literature and further screened by SwissADME. The obtained 25 potential toxic components of Psoraleae Fructus and 29 flavonoids in Glycyrrhizae Radix et Rhizoma were input into the SwissTargetPrediction for target predication. A total of 818 targets related to liver injury were screened out based on GeneCards and MalaCards, and 91 common targets of Psoraleae Fructus, Glycyrrhizae Radix et Rhizoma, and liver injury were obtained from Venny. STRING was applied for constructing the PPI network, and Metascape for analyzing the biological processes and signaling pathways that common targets participated in. Cytoscape was used to construct the component-target-disease network and component-target-pathway network for Glycyrrhizae Radix et Rhizoma against Psoraleae Fructus-induced liver injury. The predicted core targets were proto-oncogene tyrosine-protein kinase(SRC), phosphatidylinositol 4,5-bisphosphate 3-kinase subunit alpha(PIK3 CA), RAC-alpha serine/threonine-protein kinase(AKT1), etc, with PI3 K-AKT signaling pathway, MAPK signaling pathway, apoptosis, Toll-like receptor signaling pathway, and NF-κB signaling pathway mainly involved. Following the scree-ning of the main toxic and pharmacodynamic components, the pharmacodynamic effects were investigated by cell experiments. The results showed that licochalcone A was mainly responsible for alleviating coryfolin-induced liver injury, licochalcone B for coryfolin-and psoralidin-induced liver injury, and echinatin for corylifolinin-and bakuchiol-induced liver injury. The preliminary revealing of the alleviating effect of Glycyrrhizae Radix et Rhizoma on Psoraleae Fructus-induced liver injury and the prediction of related mechanisms will provide reference for further mechanism research and reasonable clinical compatibility.
