Mechanism of lung and intestine combination therapy in treatment of acute lung injury by inhibiting inflammatory response based on NF-κB/NLRP3 signaling pathway and alveolar macrophage activation.
10.19540/j.cnki.cjcmm.20211027.401
- Author:
Yu-le KOU
1
;
Wen-Ba WANG
2
;
Shu-Guang YAN
2
;
Jing-Tao LI
3
;
Jie SHI
1
;
Yi HUI
2
Author Information
1. Department of Respiratory, Affiliated Hospital of Shannxi University of Chinese Medicine Xianyang 712000, China.
2. College of Basic Medicine, Shannxi University of Chinese Medicine Xianyang 712046, China.
3. Department of Infectious Disease, Affiliated Hospital of Shannxi University of Chinese Medicine Xianyang 712000, China.
- Publication Type:Journal Article
- Keywords:
Dachengqi Decoction;
Mahuang Decoction;
NF-κB/NLRP3 signaling pathway;
acute lung injury;
inflammatory response;
lung and intestine combination therapy
- MeSH:
Acute Lung Injury/genetics*;
Animals;
Drugs, Chinese Herbal;
Intestines;
Lipopolysaccharides;
Lung/metabolism*;
Macrophage Activation;
NF-kappa B/metabolism*;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
Rats;
Signal Transduction
- From:
China Journal of Chinese Materia Medica
2022;47(1):151-158
- CountryChina
- Language:Chinese
-
Abstract:
Lung and intestine combination therapy(LICT) is effective in the treatment of acute lung injury(ALI). In this study, the combination of Mahuang Decoction and Dachengqi Decoction(hereinafter referred to as the combination), a manifestation of LICT, was employed to explore the effect of nuclear factor kappaB(NF-κB)/nucleotide binding oligomerization domain-like receptors-3(NLRP3) pathway and alveolar macrophage activation on the lung inflammation in rats with ALI, for the purpose of elucidating the mechanism of LICT in treating ALI. After the modeling of ALI with limpolysaccharide(LPS, ip), rats were respectively given(ig) the combination at 10, 7.5, and 5 g·kg~(-1)(high-dose, medium-dose, and low-dose LICT groups, separately), once every 8 h for 3 times. Haematoxylin-eosin(HE) staining was used to observe the histopathological changes of lung tissue, followed by the scoring of inflammation. Immunohistochemistry was applied to detect alveolar macrophage activation, enzyme-linked immunosorbent assay(ELISA) was applied to detect the serum content of tumor necrosis factor-α(TNF-α) and interleukin-18(IL-18), Western blot was applied to detect the protein expression of phosphorylated-nuclear factor kappaB p65(p-NF-κB p65), nuclear factor kappaB p65(NF-κB p65), phosphorylated-inhibitor kappaB alpha(p-IκBα), inhibitor kappaB alpha(IκBα), and NLRP3 in lung tissue, and quantitative reverse transcription-PCR(qRT-PCR) was applied to detect the mRNA expression of TNF-α, IL-18, NLRP3, and NF-κB p65 in lung tissue. The results showed that LICT groups demonstrated lung injury relief, decrease in inflammation score, alleviation of alveolar macrophage activation, significant decline in serum content of inflammatory factors TNF-α and IL-18, and decrease of the protein expression of p-NF-κB p65/NF-κB p65, p-IκBα/IκBα, and NLRP3, and mRNA expression of TNF-α, IL-18, NLRP3, and NF-κB p65 in lung tissue. In summary, LICT has definite therapeutic effect on ALI. The mechanism is that it inhibits alveolar macrophage activation by suppressing NF-κB/NLRP3 signaling pathway, thereby reducing the activation and release of inflammatory factors and finally inhibiting inflammation.
