Extracts of Poria cocos polysaccharides improves alcoholic liver disease in mice via CYP2E1 and NF-κB inflammatory pathways.
10.19540/j.cnki.cjcmm.20210930.402
- Author:
Yue-Hang JIANG
1
;
Yue ZHANG
1
;
Yan-Yan WANG
1
;
Wen-Xin ZHANG
1
;
Meng-Wen WANG
1
;
Chao-Qun LIU
1
;
Dai-Yin PENG
2
;
Nian-Jun YU
1
;
Lei WANG
3
;
Wei-Dong CHEN
3
Author Information
1. Anhui University of Chinese Medicine Hefei 230012, China.
2. Anhui University of Chinese Medicine Hefei 230012, China Anhui Province Key Laboratory of Chinese Medicinal Formula Hefei 230012, China.
3. Anhui University of Chinese Medicine Hefei 230012, China Anhui Province Key Laboratory of Chinese Medicinal Formula Hefei 230012, China Anhui Province Key Laboratory of Traditional Chinese Medicine Decoction Pieces of New Manufacturing Technology Hefei 230012, China Institute of Pharmaceutics, Anhui Academy of Chinese Medicine Hefei 230012, China.
- Publication Type:Journal Article
- Keywords:
CYP2E1;
NF-κB;
Poria cocos polysaccharides;
alcoholic liver disease
- MeSH:
Animals;
Cytochrome P-450 CYP2E1/pharmacology*;
Liver;
Liver Diseases, Alcoholic/pathology*;
Male;
Mice;
NF-kappa B/metabolism*;
Plant Extracts/pharmacology*;
Polysaccharides/pharmacology*;
Wolfiporia
- From:
China Journal of Chinese Materia Medica
2022;47(1):134-140
- CountryChina
- Language:Chinese
-
Abstract:
The present study investigated the effect of extract of Poria cocos polysaccharides(PCP) on cytochrome P450 2 E1(CYP2 E1) and nuclear factor κB(NF-κB) inflammatory signaling pathways in alcoholic liver disease(ALD) mice and explored its protective effect and mechanism. Sixty male C57 BL/6 N mice of SPF grade were randomly divided into a control group, a model group, a positive drug group(bifendate, 200 mg·kg~(-1)), and high-(200 mg·kg~(-1)) and low-dose(50 mg·kg~(-1)) PCP groups. Gao-binge mo-del was induced and the mice in each group were treated correspondingly. Liver morphological and pathological changes were observed and organ index was calculated. Serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) were detected. Malondialdehyde(MDA) and superoxide dismutase(SOD) in liver tissues were detected by assay kits. The levels of interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were detected by ELISA. The activation of macrophages was observed by immunofluorescence staining and protein expression of CYP2 E1, Toll-like receptor 4(TLR4), NF-κB p65, and phosphorylated NF-κB p65(p-NF-κB p65) were analyzed by Western blot. The ALD model was properly induced. Compared with the model group, the PCP groups significantly improved the pathological injury of liver tissues. Immunofluorescence staining revealed that compared with the model group, the groups with drug intervention showed decreased macrophages in liver tissues. Additionally, the PCP groups showed reduced ALT, AST, MDA, IL-6, and TNF-α(P<0.05), and potentiated activity of SOD(P<0.01). PCP extract has the protective effect against alcoholic liver injury in mice, and the underlying mechanism may be related to the regulation of the expression of CYP2 E1 and inhibition of TLR4/NF-κB inflammatory signaling pathway to reduce oxidative stress and inflammatory injury, thereby inhibiting the development of ALD.
