Preparation and intestinal absorption of Panax notoginseng saponins chitosan nanoparticles.
10.19540/j.cnki.cjcmm.20210412.302
- Author:
Peng-Fei XU
1
;
Rui ZHANG
1
;
Zhi-Yu GUAN
1
;
Si-Hui LI
1
;
Dong-Yan ZHOU
1
;
Sheng JIANG
1
;
Jin-Hui FAN
1
;
Peng XU
1
;
Wei-Feng ZHU
1
Author Information
1. School of Pharmacy, Jiangxi University of Chinese Medicine Nanchang 330004, China.
- Publication Type:Journal Article
- Keywords:
Panax notoginseng saponins(PNS);
characterization;
chitosan;
intestinal absorption;
oral nanoparticles
- MeSH:
Animals;
Chitosan/pharmacology*;
Intestinal Absorption;
Nanoparticles;
Panax notoginseng/chemistry*;
Rats;
Saponins/pharmacology*
- From:
China Journal of Chinese Materia Medica
2022;47(1):95-102
- CountryChina
- Language:Chinese
-
Abstract:
In this experiment, Panax notoginseng saponins chitosan nanoparticles(PNS-NPs) were prepared by self-assembly and their appearance, particle size, encapsulation efficiency, drug loading, polydispersity index(PDI), Zeta potential, and microstructure were characterized. The prepared PNS-NPs were intact in structure, with an average particle size of(209±0.258) nm, encapsulation efficiency of 42.34%±0.28%, a drug loading of 37.63%±0.85%, and a Zeta potential of(39.8±3.122) mV. The intestinal absorption of PNS-NPs in rats was further studied. The established HPLC method of PNS was employed to investigate the effects of pH, perfusion rate, and different drugs(PNS raw materials, Xuesaitong Capsules, and PNS-NPs). The absorption rate constant(K_a) and apparent permeability coefficient(P_(app)) in the duodenum, jejunum, ileum, and colon were calculated and analyzed. As illustrated by the results, the intestinal absorption of PNS-NPs was increased in the perfusion solution at pH 6.8(P<0.05), and perfusion rate had no significant effect on the K_a and P_(app) of PNS-NPs. The intestinal absorption of PNS-NPs was significantly different from that of PNS raw materials and Xuesaitong Capsules(P<0.05), and the intestinal absorption of PNS-NPs was significantly improved.
