Transcriptome-wide identification and expression pattern analysis for Dof gene family in Panax ginseng from Jilin.
10.19540/j.cnki.cjcmm.20211025.103
- Author:
Kang-Yu WANG
1
;
Yan-Xiu GUO
1
;
Chong MA
1
;
Ming-Zhu ZHAO
1
;
Feng-Hua LI
2
;
Mei-Ping ZHANG
1
;
Yi WANG
1
Author Information
1. College of Life Science, Jilin Agricultural University Changchun 130118, China Jilin Engineering Research Center for Ginseng Genetic Resources Development and Utilization Changchun 130118, China.
2. Teaching Department,Heilongjiang Radio and Television University Harbin 150080, China.
- Publication Type:Journal Article
- Keywords:
Dof(DNA binding with one finger) gene family;
Panax ginseng;
functional gene;
gene expression
- MeSH:
Gene Expression Profiling/methods*;
Gene Expression Regulation, Plant;
Ginsenosides;
Panax;
Plant Proteins/metabolism*;
Plant Roots/metabolism*;
Transcriptome
- From:
China Journal of Chinese Materia Medica
2022;47(1):62-71
- CountryChina
- Language:Chinese
-
Abstract:
Dof(DNA binding with one finger), a unique class of transcription factors in plants, play an important role in seed development, tissue differentiation, and metabolic regulation. To identify the number and function of Dof gene family members in Panax ginseng, this study identified the members of Dof gene family in P. ginseng and systematically analyzed their structures, evolution, functional differentiation, expression patterns, and interactions using bioinformatics methods at the transcriptome level. At the same time, the association analysis of Dof genes from P. ginseng with key enzyme genes for ginsenoside synthesis was carried out to screen the candidate PgDof genes involved in the regulation of ginsenoside biosynthesis. The results showed that there were 54 genes belonging to the Dof gene family in P. ginseng from Jilin. All PgDof genes had Zf-Dof conserved motifs, implying that they were evolutionarily conserved and could be divided into five groups. Expression pattern analysis confirmed that the expression of PgDof gene family members in different tissues, different year-old P. ginseng, and different farm varieties varied significantly. Simultaneously, as revealed by "gene-saponin content" and "gene-gene" linkage analysis, an important candidate PgDof14-1 gene involved in the regulation of ginsenoside biosynthesis was obtained. From the established genetic transformation system of this gene in the hairy roots of P. ginseng, a positive hairy root clone was determined. This study has laid a theoretical foundation for the study of Dof gene family in P. ginseng.
