Effect of Xuanfu Daizhe decoction on stemness of esophageal cancer cells.
10.12047/j.cjap.6228.2022.012
- Author:
Yuan MA
1
;
Jing XUN
2
;
Bo-Tao WANG
1
;
Di-Hua LI
2
;
Qi ZHANG
2
;
Zhen-Yu WANG
3
;
Yu WU
4
Author Information
1. Graduate School, Tianjin Medical University, Tianjin 300070.
2. Tianjin Key Laboratory of Acute Abdomen Disease Associated Organ Injury and ITCWM Repair, Nankai Clinical College, Tianjin Medical University, Tianjin 300100.
3. Department of Hepatobiliary Surgery, Nankai Clinical College, Tianjin Medical University, Tianjin 300100.
4. Department of Gastrointestinal Surgery, Nankai Clinical College, Tianjin Medical University, Tianjin 300100, China.
- Publication Type:Randomized Controlled Trial, Veterinary
- Keywords:
Xuanfu Daizhe decoction;
cancer stem cells;
cell culture;
esophageal cancer;
mice;
stemness of cancer cells
- MeSH:
Animals;
Esophageal Neoplasms/pathology*;
Mice;
Mice, Nude;
Proto-Oncogene Proteins c-akt;
Transcription Factors
- From:
Chinese Journal of Applied Physiology
2022;38(1):62-67
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of Xuanfu Daizhe decoction on the stemness of esophageal cancer cells. Methods: The BALB/c nude mice were randomly divided into the control group and experimental group, 5 mice in each group, which were continuously administered with normal saline and Xuanfu Daizhe decoction (9.89 g/kg) by gastrogavage, respectively. Human esophageal carcinoma cells ECA-109 (5×106) were subcutaneously injected into the mice on the 8th day. Tumor volume was measured twice a week. The mice were sacrificed 4 weeks after injection, and the tumor tissue and mouse serum were collected. The expressions of the major stemness-regulating transcription factors, i.e., NANOG, OCT4 and SOX2, were detected by RT-qPCR, Western Blot and immunohistochemistry. ECA-109 cells were treated with 10% fetal bovine serum and serum from the above two groups of mice for 48 hours respectively, and three replicate wells were set in each group, and the expressions of NANOG, OCT4, SOX2 and the levels of AKT and p-AKT were detected by RT-qPCR and Western Blot, respectively. ALDH activity in tumor cells was detected by flow cytometry; the number of spheroids of tumor cells was detected by the spheroidization experiment. Results: Compared with the control group, the growth and size of esophageal cancer tumors were significantly inhibited by Xuanfu Daizhe Decoction; the expressions of NANOG, OCT4, SOX2, the ALDH activity, the number of spheroids, and the levels of AKT and phosphorylated AKT (p-AKT) in esophageal cancer cells were significantly reduced by Xuanfu Daizhe Decoction both in vivo and in vitro. Conclusion: Xuanfu Daizhe Decoction inhibits the stemness of esophageal cancer cells, it may be a potentially effective drug for the treatment of esophageal cancer and provides a theoretical basis for the exploration of new effective drugs for the treatment of esophageal cancer.
