Time dependent expression profiling of PTK2B and its relationship with Aβ, Tau and LRP-1 in hippocampus and blood of APPswe/PS1dE9 double-transgenic mouse.
10.10247/j.cjap.6191.2022.004
- Author:
Kai-Min HAO
1
;
Zhen LIU
1
;
Hao-Yu WANG
1
;
Wen-Xiu QI
1
Author Information
1. Fenyang College of Shanxi Medical University, Fenyang 032200, China.
- Publication Type:Journal Article
- Keywords:
APP/PS1 transgenic mice;
Alzheimer's disease;
PTK2B;
beta amyloid protein;
low density lipoprotein receptor-related protein-1
- MeSH:
Alzheimer Disease/metabolism*;
Amyloid beta-Peptides;
Amyloid beta-Protein Precursor/genetics*;
Animals;
Focal Adhesion Kinase 2/metabolism*;
Hippocampus/metabolism*;
Low Density Lipoprotein Receptor-Related Protein-1;
Maze Learning;
Mice;
Mice, Inbred C57BL;
Mice, Transgenic;
RNA, Messenger
- From:
Chinese Journal of Applied Physiology
2022;38(1):17-24
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To uncover the time-dependent expression pattern of ptk2b gene and ptk2b-encoded protein, protein tyrosine kinase 2 beta(PTK2B), in the brain tissues of transgenic animal models of Alzheimer's disease (AD) and its relationship with the levels of Aβ1-42, phosphorylation of Tau (p-Tau) and low density lipoprotein receptor-related protein-1(LRP-1) in blood and brain tissues. Methods: In this study, 5-, 10- and 15-month-old APPswe/PS1dE9 double-transgenic mice harboring the genotype of AD confirmed by the gene test were divided into the 5-, 10- and 15-month-old experiment groups, and simultaneously, age-matched C57BL/6J mice were placed into the corresponding control groups, with 8 mice in each group. All mice were subjected to the Morris Water Maze for test of cognitive and behavioral ability. Expression profiles of PTK2B, Aβ1-42, p-Tau/Tau and LRP-1 in the hippocampus or blood of mice were quantified by using the immunohistochemistry staining, Western blot or enzyme-linked immunosorbent assay (ELISA), while the mRNA expression of ptk2b in the hippocampus was quantified by using the real-time quantitative polymerase chain reaction (qRT-PCR). Results: Results of experiment groups demonstrated that as mice aged, the expression levels of PTK2B, ptk2b mRNA, Aβ1-42 and p-Tau/Tau in the hippocampus were increased, and the expression of LRP-1 was decreased gradually. While in the blood, the level of Aβ1-42 was decreased, and the cognitive and behavioral ability was decreased in an age-dependent manner (all P＜ 0.05). However, comparisons among the control groups, only the age-dependent downregulation of LRP-1 were observed in hippocampus(P＜0.05), but other indicators had no significant differences (P＞0.05). Conclusion: In the hippocampus of APP/PS1 double-transgenic mice, the expressions of PTK2B, Aβ1-42 and p-Tau/Tau are upregulated, LRP-1 is downregulated, while cognitive and behavioral ability is decreased, and such changes are presented in a time-dependent manner.
