(+)-corynoline Regulates the Proliferation,Stemness and Apoptosis of Triple Negative Breast Cancer Cells.
10.3881/j.issn.1000-503X.14366
- Author:
Shi-Lin LI
1
;
Xiang-Yi KONG
1
;
Yi FANG
1
Author Information
1. National Cancer Center,National Clinical Research Center for Cancer,Department of Breast Surgical Oncology of Cancer Hospital,CAMS and PUMC,Beijing 100021,China.
- Publication Type:Journal Article
- Keywords:
(+)-corynoline;
apoptosis;
oxidative phosphorylation pathway;
proliferation;
triple negative breast cancer
- MeSH:
Apoptosis;
Berberine Alkaloids;
Breast Neoplasms;
Cell Line, Tumor;
Cell Movement;
Cell Proliferation;
Female;
Humans;
Triple Negative Breast Neoplasms/metabolism*
- From:
Acta Academiae Medicinae Sinicae
2022;44(2):244-252
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the performance and mechanism of(+)-corynoline in treating triple negative breast cancer MDA-MB-436 cells and thus provide an option for the development of drugs against this cancer. Methods The viability,proliferation,apoptosis and migration/invasion of MDA-MB-436 cells treated with(+)-corynoline were detected by CCK-8 assay,colony formation assay,flow cytometry and Transwell assay,respectively.Furthermore,Western blotting was employed to determine the expression of related proteins,and RNA-Seq was performed for the MDA-MB-436 cells treated with(+)-corynoline. Results (+)-corynoline inhibited the proliferation and stemness and promoted the apoptosis of MDA-MB-436 cells.Further,(+)-corynoline may activate the oxidative phosphorylation pathway to play a role in inhibiting triple negative breast cancer. Conclusion (+)-corynoline can inhibit triple negative breast cancer cells,which helps to address the poor efficacy of existing chemotherapeutics and facilitate the development of drugs against this cancer.
