Identification and verification of key salt-tolerant amino acid sites of banana MaNHX5.

Ya XU; Mengxin Teng; Fei Qiao; Xingyu Jiang; Yang Zhou; Xinguo LI

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Identification and verification of key salt-tolerant amino acid sites of banana MaNHX5.

Author: Ya XU ¹ ; Mengxin TENG ¹ ; Fei QIAO ² ; Xingyu JIANG ³ ; Yang ZHOU ¹ ; Xinguo LI ¹
Author Information

1. School of Horticulture, Hainan University, Haikou 570228, Hainan, China.
2. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou 570100, Hainan, China.
3. College of Tropical Crops, Hainan University, Haikou 570228, Hainan, China.
Publication Type:Journal Article
Keywords: NHX gene; banana; salt tolerance; site-directed mutagenesis
MeSH: Amino Acids/metabolism*; Gene Expression Regulation, Plant; Musa/metabolism*; Plant Proteins/metabolism*; Plants, Genetically Modified; Saccharomyces cerevisiae/metabolism*
From: Chinese Journal of Biotechnology 2022;38(5):1946-1952
CountryChina
Language:Chinese
Abstract: In order to improve the salt tolerance of banana NHX genes, we cloned a MaNHX5 gene from Musa acuminata L. AAA group and predicted the key salt-tolerant amino acid sites and mutant protein structure changes of MaNHX5 by using bioinformatics tools. The 276-position serine (S) of MaNHX5 protein was successfully mutated to aspartic acid (D) by site-directed mutagenesis, and the AXT3 salt-sensitive mutant yeast was used for a functional complementation test. The results showed that after the mutated MaNHX5 gene was transferred to AXT3 salt-sensitive mutant yeast, the salt tolerance of the mutant yeast was significantly improved under 200 mmol/L NaCl treatment. It is hypothesized that Ser276 of MaNHX5 protein plays an important role in the transport of Na⁺ across the tonoplast.