Transformation mechanism of carbon tetrachloride and the associated micro-ecology in landfill cover, a typical functional layer zone.

Yongqiong Wang; Zhilin Xing; Shangjie Chen; Xia SU; Kun Cao; Ludan Cao; Shushu Liao; Langlang Dong; Shuo AI; Tiantao Zhao

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Transformation mechanism of carbon tetrachloride and the associated micro-ecology in landfill cover, a typical functional layer zone.

Author: Yongqiong WANG ¹ ; Zhilin XING ¹ ; Shangjie CHEN ¹ ; Xia SU ¹ ; Kun CAO ¹ ; Ludan CAO ¹ ; Shushu LIAO ¹ ; Langlang DONG ¹ ; Shuo AI ² ; Tiantao ZHAO ¹
Author Information

1. School of Chemistry and Chemical Engineering, Chongqing University of Technology, Chongqing 400054, China.
2. Chongqing Shiji Ecological Environment Technology Co., Ltd., Chongqing 404100, China.
Publication Type:Journal Article
Keywords: carbon tetrachloride; function layer zone; functional microorganism; hypoxic zone; landfill cover
MeSH: Bacteria/metabolism*; Biodegradation, Environmental; Carbon Tetrachloride/metabolism*; Methane/metabolism*; Waste Disposal Facilities
From: Chinese Journal of Biotechnology 2022;38(5):1874-1888
CountryChina
Language:Chinese
Abstract: Landfill is one of the important sources of carbon tetrachloride (CT) pollution, and it is important to understand the degradation mechanism of CT in landfill cover for better control. In this study, a simulated landfill cover system was set up, and the biotransformation mechanism of CT and the associated micro-ecology were investigated. The results showed that three stable functional zones along the depth, i.e., aerobic zone (0-15 cm), anoxic zone (15-45 cm) and anaerobic zone (> 45 cm), were generated because of long-term biological oxidation in landfill cover. There were significant differences in redox condition and microbial community structure in each zone, which provided microbial resources and favorable conditions for CT degradation. The results of biodegradation indicated that dechlorination of CT produced chloroform (CF), dichloromethane (DCM) and Cl^- in anaerobic and anoxic zones. The highest concentration of dechlorination products occurred at 30 cm, which were degraded rapidly in aerobic zone. In addition, CT degradation rate was 13.2-103.6 μg/(m²·d), which decreased with the increase of landfill gas flux. The analysis of diversity sequencing revealed that Mesorhizobium, Thiobacillus and Intrasporangium were potential CT-degraders in aerobic, anaerobic and anoxic zone, respectively. Moreover, six species of dechlorination bacteria and eighteen species of methanotrophs were also responsible for anaerobic transformation of CT and aerobic degradation of CF and DCM, respectively. Interestingly, anaerobic dechlorination and aerobic transformation occurred simultaneously in the anoxic zone in landfill cover. Furthermore, analysis of degradation mechanism suggested that generation of stable anaerobic-anoxic-aerobic zone by regulation was very important for the harmless removal of full halogenated hydrocarbon in vadose zone, and the increase of anoxic zone scale enhanced their removal. These results provide theoretical guidance for the removal of chlorinated pollutants in landfills.