Prevention and control of antimicrobial resistance using CRISPR-Cas system: a review.

Chenyu Wang; Zhizhi Liu; Biao Tang; Hua Yang; Dongchang Sun

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Prevention and control of antimicrobial resistance using CRISPR-Cas system: a review.

Author: Chenyu WANG ¹ ; Zhizhi LIU ¹ ; Biao TANG ² ; Hua YANG ² ; Dongchang SUN ¹
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1. College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, Zhejiang, China.
2. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China.
Publication Type:Review
Keywords: CRISPR-Cas system; antibiotic resistance genes; horizontal gene transfer; multi-drug resistance
MeSH: Anti-Bacterial Agents; Bacteriophages/genetics*; CRISPR-Cas Systems; Drug Resistance, Bacterial/genetics*; Plasmids/genetics*
From: Chinese Journal of Biotechnology 2022;38(4):1432-1445
CountryChina
Language:Chinese
Abstract: Bacterial multi-drug resistance (MDR) is a global challenge in the fields of medicine and health, agriculture and fishery, ecology and environment. The cross-region spread of antibiotic resistance genes (ARGs) among different species is one of the main cause of bacterial MDR. However, there is no effective strategies for addressing the intensifying bacterial MDR. The CRISPR-Cas system, consisting of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR associated proteins, can targetedly degrade exogenous nucleic acids, thus exhibiting high application potential in preventing and controlling bacterial MDR caused by ARGs. This review briefly introduced the working mechanism of CRISPR-Cas systems, followed by discussing recent advances in reducing ARGs by CRISPR-Cas systems delivered through mediators (e.g. plasmids, bacteriophages and nanoparticle). Moreover, the trends of this research field were envisioned, providing a new perspective on preventing and controlling MDR.