Expression of influenza B virus hemagglutinin and its immunogenicity determination.
- Author:
Chen YANG
1
;
Xiaoyu GENG
1
;
Kai YUAN
2
;
Juankun ZHANG
1
;
Haixia XIAO
2
Author Information
- Publication Type:Journal Article
- Keywords: hemagglutinin; influenza B virus; mammalian cell expression system; vaccine candidate
- MeSH: Animals; Hemagglutinin Glycoproteins, Influenza Virus/genetics*; Hemagglutinins/genetics*; Influenza B virus/metabolism*; Influenza Vaccines/genetics*; Mammals/metabolism*; Mice; Mice, Inbred BALB C
- From: Chinese Journal of Biotechnology 2022;38(3):1112-1123
- CountryChina
- Language:Chinese
- Abstract: Influenza B virus is one of the causes for seasonal influenza, which can account for serious illness or even death in some cases. We tested the expression of extracellular domain of hemagglutinin (HA-ecto) of influenza B viruses in mammalian cells, and then determined the immunogenicity of HA-ecto in mice. The gene sequence encoding influenza B virus HA-ecto, foldon sequence, and HIS tag was optimized and inserted into pCAGGS vector. The opening reading frame (ORF) of neuraminidase was also cloned into pCAGGS. The pCAGGS-HA-ecto and pCAGGS-NA were co-transfected into 293T cells using linear polyethylenimine. Cell supernatant after transfection was collected after 96 h, and the secreted trimmeric HA-ecto protein was purified by nickel ion affinity chromatography and size exclusion chromatography. Subsequently, the mice were immunized with HA-ecto protein, and the corresponding antibody titers were detected by ELISA and hemagglutination inhibition (HAI) assays. The results showed that soluble trimeric HA-ecto protein could be obtained using mammalian cell expression system. Moreover, trimeric HA-ecto protein, in combination with the adjuvant, induced high levels of ELISA and HAI antibodies against homogenous and heterologous antigens in mice. Thus, the soluble HA-ecto protein expressed in mammalian cells could be used as a recombinant subunit vaccine candidate for influenza B virus.
