Regulating the structure of bacterial cellulose by altering the expression of <i>bcsD</i> using CRISPR/dCas9.

Longhui Huang; Xuejing LI; Xuewen Sun; Xu Wang; Yitong Wang; Shiru Jia; Cheng Zhong

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Regulating the structure of bacterial cellulose by altering the expression of bcsD using CRISPR/dCas9.

Author: Longhui HUANG ¹ ; Xuejing LI ¹ ; Xuewen SUN ¹ ; Xu WANG ¹ ; Yitong WANG ¹ ; Shiru JIA ¹ ; Cheng ZHONG ¹
Author Information

1. State Key Laboratory of Food Nutrition & Safety, Tianjin University of Science & Technology, Tianjin 300457, China.
Publication Type:Journal Article
Keywords: Gluconacetobacter xylinus; CRISPR/dCas9; bacterial cellulose; bcsD
MeSH: Cellulose/chemistry*; Clustered Regularly Interspaced Short Palindromic Repeats; Fermentation; Gluconacetobacter xylinus/metabolism*
From: Chinese Journal of Biotechnology 2022;38(2):772-779
CountryChina
Language:Chinese
Abstract: Gluconacetobacter xylinus is a primary strain producing bacterial cellulose (BC). In G. xylinus, BcsD is a subunit of cellulose synthase and is participated in the assembly process of BC. A series of G. xylinus with different expression levels of the bcsD gene were obtained by using the CRISPR/dCas9 technique. Analysis of the structural characteristics of BC showed that the crystallinity and porosity of BC changed with the expression of bcsD. The porosity varied from 59.95%-84.05%, and the crystallinity varied from 74.26%-93.75%, while the yield of BC did not decrease significantly upon changing the expression levels of bcsD. The results showed that the porosity of bacterial cellulose significantly increased, while the crystallinity was positively correlated with the expression of bcsD, when the expression level of bcsD was below 55.34%. By altering the expression level of the bcsD gene, obtaining BC with different structures but stable yield through a one-step fermentation of G. xylinus was achieved.