Engineering Saccharomyces cerevisiae for efficient production of glucaric acid.
- Author:
Jie LI
1
;
Yunying ZHAO
1
;
Yu DENG
1
Author Information
- Publication Type:Journal Article
- Keywords: Saccharomyces cerevisiae; fusional expression; glucaric acid; inositol transporter; metabolic engineering
- MeSH: Fermentation; Glucaric Acid/metabolism*; Inositol Oxygenase/genetics*; Metabolic Engineering; Saccharomyces cerevisiae/metabolism*
- From: Chinese Journal of Biotechnology 2022;38(2):705-718
- CountryChina
- Language:Chinese
- Abstract: As an important dicarboxylic acids existing in nature, glucaric acid has been widely used in medical, health, and polymer materials industry, therefore it is considered as one of the "top value-added chemicals from biomass". In this study, using Saccharomyces cerevisiae as a chassis microorganism, the effects of overexpression of myo-inositol transporter Itr1, fusional expression of inositol oxygenase MIOX4 and uronate dehydrogenase Udh, and down-expression of glucose-6-phosphate dehydrogenase gene ZWF1 on the glucaric acid production were investigated. The results showed that the yield of glucaric acid was increased by 26% compared with the original strain Bga-3 under shake flask fermentation after overexpressing myo-inositol transporter Itr1. The yield of glucaric acid was increased by 40% compared with Bga-3 strain by expressing the MIOX4-Udh fusion protein. On these basis, the production of glucaric acid reached 5.5 g/L, which was 60% higher than that of Bga-3 strain. In a 5 L fermenter, the highest yield of glucaric acid was 10.85 g/L, which was increased 80% compared with that of Bga-3 strain. The application of the above metabolic engineering strategy improved the pathway efficiency and the yield of glucaric acid, which may serve as a reference for engineering S. cerevisiae to produce other chemicals.
