Biosynthesis and regulatory mechanism of acarbose and its structural analogs: a review.
- Author:
Yuanshan WANG
1
;
Kelei DAI
1
;
Kaxi XIE
1
;
Chunyue WENG
1
Author Information
- Publication Type:Review
- Keywords: Actinoplanes sp.; acarbose; analogs; biosynthesis; regulation
- MeSH: Acarbose/metabolism*; Diabetes Mellitus, Type 2/drug therapy*; Genetic Techniques; Humans
- From: Chinese Journal of Biotechnology 2022;38(2):605-619
- CountryChina
- Language:Chinese
- Abstract: Acarbose is widely used as α-glucosidase inhibitor in the treatment of type Ⅱ diabetes. Actinoplanes sp. is used for industrial production of acarbose. As a secondary metabolite, the biosynthesis of acarbose is quite complex. In addition to acarbose, a few acarbose structural analogs are also accumulated in the culture broth of Actinoplanes sp., which are hard to remove. Due to lack of systemic understanding of the biosynthesis and regulation mechanisms of acarbose and its structural analogs, it is difficult to eliminate or reduce the biosynthesis of the structural analogs. Recently, the advances in omics technologies and molecular biology have facilitated the investigations of biosynthesis and regulatory mechanisms of acarbose and its structural analogs in Actinoplanes sp.. The genes involved in the biosynthesis of acarbose and its structural analogs and their regulatory mechanism have been extensively explored by using bioinformatics analysis, genetic manipulation and enzymatic characterization, which is summarized in this review.
