The recent advances in developing gene editing and expression tools and the synthesis of natural products in Yarrowia lipolytica.
- Author:
Jinhong ZHANG
1
;
Zhiyong CUI
1
;
Qingsheng QI
1
;
Jin HOU
1
Author Information
- Publication Type:Review
- Keywords: Yarrowia lipolytica; expression tools; metabolic engineering; synthesis of natural products; unconventional yeast
- MeSH: Biological Products/metabolism*; Gene Editing; Metabolic Engineering; Polyketides/metabolism*; Yarrowia/metabolism*
- From: Chinese Journal of Biotechnology 2022;38(2):478-505
- CountryChina
- Language:Chinese
- Abstract: Yarrowia lipolytica, as an important oleaginous yeast, has been widely used in metabolic engineering. Y. lipolytica is considered as an ideal host for the production of natural products such as terpenes, polyketides and flavonoids, due to its ability to utilize a variety of hydrophobic substrates, high stress tolerance to acid and salt, high flux of tricarboxylic acid cycle and the ability in providing abundant the common precursor acetyl-CoA. Recently, more and more tools for genetic editing, gene expression and regulation has been developed in Y. lipolytica, which facilitate the metabolic engineering of Y. lipolytica for bio-manufacturing. In this review, we summarized the recent progresses in developing gene expression and natural product synthesis in Y. lipolytica, and also discussed the challenges and possible solutions in heterologous synthesis of natural products in this yeast.
