Identification and analysis of the TALE transcription factor family in radish.

Wei Zhao; Xixiang LI; Haiping Wang; Huixia Jia; Jiangping Song; Wenlong Yang; Xiaohui Zhang

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Identification and analysis of the TALE transcription factor family in radish.

Author: Wei ZHAO ¹ ; Xixiang LI ¹ ; Haiping WANG ¹ ; Huixia JIA ¹ ; Jiangping SONG ¹ ; Wenlong YANG ¹ ; Xiaohui ZHANG ¹
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1. Beijing Research Station of Vegetable Crop Gene Resource and Germplasm Enhancement, Ministry of Agriculture and Rural Affairs of People's Republic of China, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture and Rural Affairs of People's Republic of China, Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China.
Publication Type:Journal Article
Keywords: Raphanus sativus; TALE gene family; evolutionary analysis; gene structure; tissue expression
MeSH: Amino Acids; Arabidopsis/metabolism*; Gene Expression Regulation, Plant; Phylogeny; Plant Proteins/metabolism*; Raphanus/metabolism*; Transcription Factors/metabolism*
From: Chinese Journal of Biotechnology 2022;38(1):343-358
CountryChina
Language:Chinese
Abstract: Three-amino acid loop extension (TALE) transcription factors play important roles in plant growth and cell differentiation. There are plenty of studies on TALE transcription factors in several model plants, but not in radish (Raphanus sativas). A genome-wide bioinformatics analysis identified 33 TALE family genes in the Xiang-Ya-Bai (XYB) radish, These genes, are distributed on nine chromosomes and all contain 4-6 exons. The 33 TALE genes in radish showed a co-linearity relationship with the 17 homologous genes in Arabidopsis thaliana. Moreover, a large number of stress response cis-elements were found in the promoter regions of these genes. Expression analysis showed that four genes in the BELL subfamily were highly expressed in roots, and two genes in the KNOX subfamily were highly expressed in shoots of bolting plants and callus. All radish TALE genes contain sequences encoding the conserved HOX domain, except for the gene RSA10037940, which is homologous to Arabidopsis KNATM. The deduced 3D structures of the TALE proteins irrespective of subtypes are highly similar. All the encoded proteins were weakly acidic and hydrophilic. The radish TALE gene family is relatively evolutionarily conserved, which was consistent with results from Arabidopsis, but quite different from that of rice. This study provides important clues for studying the biological functions of TALE transcription factors in radish.