Cloning, structure analysis and functional verification of <i>MYB10</i> in <i>Ribes</i> L.

Qiuying Feng; Xue Liu; Linlin Yang; Zeyuan FU; Qijiang XU

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Cloning, structure analysis and functional verification of MYB10 in Ribes L.

Author: Qiuying FENG ¹ ; Xue LIU ¹ ; Linlin YANG ¹ ; Zeyuan FU ¹ ; Qijiang XU ¹
Author Information

1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration (Northeast Forestry University), Ministry of Education, Harbin 150040, Heilongjiang, China.
Publication Type:Journal Article
Keywords: MYB10; Ribes L.; expression level; functional verification; gene cloning
MeSH: Anthocyanins; Cloning, Molecular; Fruit; Gene Expression Regulation, Plant; Phylogeny; Plant Proteins/metabolism*; Ribes/genetics*
From: Chinese Journal of Biotechnology 2022;38(1):275-286
CountryChina
Language:Chinese
Abstract: This study aims to investigate the molecular mechanism of the transcription factor MYB10, which is involved in anthocyanin biosynthesis, in different colors of Ribes L. fruitification. Rapid amplification of cDNA ends (RACE) was used to clone the MYB10 genes from Ribes nigrum L. (RnMYB10), Ribes rubrum L. (RrMYB10), and Ribes album L. (RaMYB10), respectively. Phylogenetic analysis showed that RnMYB10 and RrMYB10 were evolutionarily homologous. Real-time quantitative PCR (RT-qPCR) showed that the expression of MYB10 in the fruits of Ribes nigrum L. was higher than that of Ribes rubrum L. and much higher than that of Ribes album L. The expression of RnMYB10 and RrMYB10 increased at first and then decreased as the fruit diameter increased and the fruit color deepened (the maximum expression level was reached at 75% of the fruit color change), while the expression level of RaMYB10 was very low. Overexpression of RnMYB10 and RrMYB10 in Arabidopsis thaliana resulted in purple petioles and leaves, whereas overexpression of RaMYB10 resulted in no significant color changes. This indicates that MYB10 gene plays an important role in the coloration of Ribes L. fruit.