The application of carbohydrate binding module-<i>Thermobifida fusca</i> cutinase fusion protein in polyethylene terephthalate degradation.

Ying Zhang; Zhanzhi Liu; Guangyao LI; Xueni FU; Yucheng Zhang; Zhiyuan Wang; Yaping Tian; Jing WU

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The application of carbohydrate binding module-Thermobifida fusca cutinase fusion protein in polyethylene terephthalate degradation.

Author: Ying ZHANG ¹ ; Zhanzhi LIU ¹ ; Guangyao LI ¹ ; Xueni FU ² ; Yucheng ZHANG ² ; Zhiyuan WANG ² ; Yaping TIAN ² ; Jing WU ¹
Author Information

1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China.
2. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Bioengineering, Jiangnan University, Wuxi 214122, Jiangsu, China.
Publication Type:Journal Article
Keywords: biodegradation; carbohydrate-binding module; cutinase; fusion protein; polyethylene terephthalate
MeSH: Carbohydrates; Carboxylic Ester Hydrolases; Polyethylene Terephthalates; Thermobifida
From: Chinese Journal of Biotechnology 2022;38(1):217-225
CountryChina
Language:Chinese
Abstract: With the development of global economy, the dramatically increased production of polyethylene terephthalate (PET) plastics has led to a remarkably increased amount of plastic waste. PET waste can be treated by landfill, incineration, or biodegradation. While landfilling and incineration may cause secondary pollution, biodegradation has since received increased attentions due to its environmental friendliness. Recent studies have indicated that the carbohydrate binding module (CBM) can effectively enhance the binding of PET degrading enzymes to PET, and consequently increasing PET degradation rate. Here we constructed a fusion protein BaCBM2-Tfuc containing the BaCBM2 from Bacillus anthraci and the cutinase Tfuc from Thermobifida fusca, by megaprimer PCR of whole plasmids (MEGAWHOP). Notabaly, the PET film degradation efficiency (at 60 ℃) of BaCBM2-Tfuc was 2.8 times that of Tfuc. This study may provide technical support for constructing fusion proteins capable of efficiently degrading PET.