Inhibition of Ciliogenesis Enhances the Cellular Sensitivity to Temozolomide and Ionizing Radiation in Human Glioblastoma Cells.

Li Wei; Wei MA; Hui Cai; Shao Peng Peng; Huan Bing Tian; Ju Fang Wang; Lan Gao; Jin Peng HE

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Inhibition of Ciliogenesis Enhances the Cellular Sensitivity to Temozolomide and Ionizing Radiation in Human Glioblastoma Cells.

Author: Li WEI ^{1
,

2} ; Wei MA ^{3
,

4} ; Hui CAI ² ; Shao Peng PENG ² ; Huan Bing TIAN ⁵ ; Ju Fang WANG ^{3
,

4} ; Lan GAO ⁵ ; Jin Peng HE ^{3
,

4}
Author Information

1. School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu, China
2. NHC Key Laboratory of Diagnosis and Therapy of Gastrointestinal Tumor, Gansu Provincial Hospital, Lanzhou 730000, Gansu, China.
3. Key Laboratory of Space Radiobiology of Gansu Province & CAS Key Laboratory of Heavy Ion Radiation Biology and Medicine, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, Gansu, China
4. University of Chinese Academy of Sciences, Beijing 100049, China.
5. School of Life Sciences, Lanzhou University, Lanzhou 730000, Gansu, China.
Publication Type:Journal Article
Keywords: Autophagy; Cellular sensitivity; DNA damage response; DNA-PK; Glioblastoma; Ionizing radiation; Primary cilia; Temozolomide
MeSH: Antineoplastic Agents, Alkylating/therapeutic use*; Brain Neoplasms/metabolism*; Cell Line, Tumor; DNA/therapeutic use*; Glioblastoma/metabolism*; Humans; Radiation, Ionizing; Temozolomide/therapeutic use*
From: Biomedical and Environmental Sciences 2022;35(5):419-436
CountryChina
Language:English
Abstract: Objective:To investigate the function of primary cilia in regulating the cellular response to temozolomide (TMZ) and ionizing radiation (IR) in glioblastoma (GBM).
Methods:GBM cells were treated with TMZ or X-ray/carbon ion. The primary cilia were examined by immunostaining with Arl13b and γ-tubulin, and the cellular resistance ability was measured by cell viability assay or survival fraction assay. Combining with cilia ablation by IFT88 depletion or chloral hydrate and induction by lithium chloride, the autophagy was measured by acridine orange staining assay. The DNA damage repair ability was estimated by the kinetic curve of γH2AX foci, and the DNA-dependent protein kinase (DNA-PK) activation was detected by immunostaining assay.
Results:Primary cilia were frequently preserved in GBM, and the induction of ciliogenesis decreased cell proliferation. TMZ and IR promoted ciliogenesis in dose- and time-dependent manners, and the suppression of ciliogenesis significantly enhanced the cellular sensitivity to TMZ and IR. The inhibition of ciliogenesis elevated the lethal effects of TMZ and IR via the impairment of autophagy and DNA damage repair. The interference of ciliogenesis reduced DNA-PK activation, and the knockdown of DNA-PK led to cilium formation and elongation.
Conclusion:Primary cilia play a vital role in regulating the cellular sensitivity to TMZ and IR in GBM cells through mediating autophagy and DNA damage repair.