Development of an approach for simultaneous determination ofmulti-mycotoxins in fresh fruits
10.19485/j.cnki.issn2096-5087.2022.06.003
- Author:
Jiaojiao XU
;
Zengxuan CAI
;
Junlin WANG
;
Meijia XU
;
Baifen HUANG
- Publication Type:Journal Article
- Keywords:
mycotoxins;
liquid chromatography-tandem mass spectrometry;
fresh fruits
- From:
Journal of Preventive Medicine
2022;34(6):555-560
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop an approach for simultaneous detection of multi-mycotoxins in fresh fruits, so as to provide technical supports for mycotoxins surveillance in fresh fruits.
Methods:Fresh fruits were collected from markets and homogenized. Then, 2 g of fresh fruits were added with 10 mL of 0.1% formic acid ( 99∶1, v/v ) in acetonitrile and wortexed for 10 min. Following extraction with 1 g of sodium chloride and 4 g of anhydrous sodium sulfate, samples were centrifuged and 5 mL of the supernatant was cleaned up with 25 mg C18. Following centrifugation, the supernatant was dried under nitrogen. The residue was dissolved in 300 μL of methanol-acetonitrile mixture solution ( 1∶1, v/v ), and mixed evenly in 700 μL of the distilled water. Samples were then eluted in gradient series of 0.1% formic acid and 5 mmol ammonium formate and methanol-acetonitrile mixture solution ( 1∶1, v/v ). The 15 mycotoxins were determined using liquid chromatography-tandem mass spectrometry ( LC-MS/MS ) with electrospray ion source (ESI+/ESI-) under multiple reaction monitoring. In addition, a matrix-matched standard curve was employed for quantitative analysis.
Results:There was a good linear relationship for 15 mycotoxins at concentrations of 0.25 to 10 ng/mL ( R2>0.992 ), the LC-MS/MS method showed the detection limits of 0.1-1.0 μg/kg, the spiked recovery rates of 71.68%-117.50%, and the relative standard deviations ( RSDs ) of 0.01%-13.60%. The detection rate of mycotoxins was 27.09% in 203 fresh fruits sold in markets.
Conclusions:The optimized LC-MS/MS method can be used for simultaneous determination of multi-mycotoxins in fresh fruits.
- Full text:新鲜水果真菌毒素多组分检测方法研究.pdf
