Antinociceptive, anti-inflammatory, and cytotoxic properties of Origanum vulgare essential oil, rich with β-caryophyllene and β-caryophyllene oxide
10.3344/kjp.2022.35.2.140
- Author:
Armenuhi MOGHROVYAN
1
;
Lilya PARSEGHYAN
;
Gohar SEVOYAN
;
Anna DARBINYAN
;
Naira SAHAKYAN
;
Monica GABOYAN
;
Zaruhi KARABEKIAN
;
Armen VOSKANYAN
Author Information
1. Department of Pharmacognosy, Yerevan State Medical University after M. Heratsi, Yerevan, Armenia
- Publication Type:Experimental Research Article
- From:The Korean Journal of Pain
2022;35(2):140-151
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Essential oils are of great interest for their analgesic and anti-inflammatory properties. We aimed to study the content of the essential oil of the Origanum vulgare of the Armenian highlands (OVA) in different periods of vegetation and to investigate its antinociceptive and anti-inflammatory effects in mice (in vivo) and cytotoxic action in cultured cells (in vitro). OVA essential oil was extracted from fresh plant material by hydro-distillation.
Methods:For OVA essential oil contents determination the gas chromatographymass spectrometry method was used. Formalin and hot plate tests and analysis of cell viability using the methyl-thiazolyl-tetrazolium (MTT) assay were used.
Results:The maximal content of β-caryophyllene and β-caryophyllene oxide in OVA essential oil was revealed in the period of blossoming (8.18% and 13.36%, correspondently). In the formalin test, 4% OVA essential oil solution (3.5 mg/mouse) exerts significant antinociceptive and anti-inflammatory effects (P = 0.003). MTT assay shows approximately 60% cytotoxicity in HeLa and Vero cells for 2.0 μL/mL OVA essential oil in media.
Conclusions:The wild oregano herb of Armenian highlands, harvested in the blossoming period, may be considered as a valuable source for developing pain-relieving preparations.
