Clinical Significance of Rab27a as a Urinary Biomarker in Patients With Bladder Cancer
10.22465/kjuo.2022.20.1.52
- Author:
Ja Yoon KU
1
;
Eu Chang HWANG
;
Chan Ho LEE
;
Kyung Hwan KIM
;
Dong Deuk KWON
;
Hong Koo HA
Author Information
1. Department of Urology, Dongnam Institite of Radiological & Medical Sciences, Busan, Korea
- Publication Type:Original Article
- From:Korean Journal of Urological Oncology
2022;20(1):52-58
- CountryRepublic of Korea
- Language:English
-
Abstract:
Purpose:The aims of this study were to investigate the clinical value of Rab27a as a urinary biomarker, and its efficiency in the prediction of bladder cancer grade.
Materials and Methods:The expression of Rab27a in urine samples of patients with bladder cancer, cell line (T-24), and tissue samples of patients with bladder cancer was estimated via quantitative reverse transcription polymerase chain reaction (qRT-PCR). The Rab27a expression level was investigated according to sex, age, and histological grade via qRT-PCR and Western blotting.
Results:Rab27a was also expressed at high levels in urine compared to cell lines and tissues from bladder cancer patients. In addition, Rab27a expression varied significantly according to tumor grade (p<0.001). Rab27a was expressed at high levels in male and elderly patients, however, there was not statistically significant.
Conclusions:Our results indicated that Rab27a is valuable as a urinary diagnostic biomarker for bladder cancer. In addition, it may serve as a predictive factor for determining bladder cancer grade.
