Effect of Ixeris dentata Nakai Extract on Nitric Oxide Production and Prostaglandin E2 Generation in LPS-stimulated RAW264.7 Cells.
- Author:
Yu Yeon JUNG
1
;
Jin Tae HONG
;
Sang Bae HAN
;
Young Hyun PARK
;
Dong Ju SON
Author Information
- Publication Type:In Vitro ; Brief Communication
- Keywords: Ixeris dentate Nakai; Inflammation; Nitric oxide; Prostaglandin E2; iNOS; COX-2
- MeSH: Asteraceae*; Cell Line; Chronic Disease; Cyclooxygenase 2; Dinoprostone*; Inflammation; Methanol; Nitric Oxide Synthase; Nitric Oxide*
- From:Immune Network 2015;15(6):325-330
- CountryRepublic of Korea
- Language:English
- Abstract: Inflammation is the basis of severe acute and chronic diseases. This study investigated the anti-inflammatory property of a crude methanol extract (MeOH-ex) and the solvent fractions of Ixeris dentata Nakai (IDN) in LPS-stimulated murine macrophage-like cell line RAW264.7. Here, we showed that the ethyl acetate fraction (EtOAc-fr) had the most potent inhibitory activity on LPS-induced nitric oxide (NO) production among the tested samples, i.e., IDN MeOH-ex and the three different solvent fractions (chloroform, n-hexane, and EtOAc). We further found that the EtOAc-fr significantly inhibited LPS-induced prostaglandin PGE2 (PGE2) generation in RAW264.7 cells. Furthermore, the treatment with EtOAc-fr effectively suppressed the expression of inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2). These results suggest that the EtOAc-fr of IDN MeOH-ex exhibits an anti-inflammatory activity in vitro by inhibiting LPS-induced NO production and PGE2 generation via suppression of iNOS and COX-2 expression.