Dimethyloxaloylglycine promotes spermatogenesis activity of spermatogonial stem cells in Bama minipigs
- Author:
Yaqi CAO
1
;
ZiFu DAI
;
Huizhen LAO
;
Huimin ZHAO
Author Information
- Publication Type:Original Article
- From:Journal of Veterinary Science 2022;23(2):e35-
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:The testis has been reported to be a naturally O 2 -deprived organ, dimethyloxaloylglycine (DMOG) can inhibit hypoxia inducible factor-1alpha (HIF-1α) subject to degradation under normal oxygen condition in cells.
Objectives:The objective of this study is to detect the effects of DMOG on the proliferation and differentiation of spermatogonial stem cells (SSCs) in Bama minipigs.
Methods:Gradient concentrations of DMOG were added into the culture medium, HIF-1αprotein in SSCs was detected by western blot analysis, the relative transcription levels of the SSC-specific genes were analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Six days post-induction, the genes related to spermatogenesis were detected by qRT-PCR, and the DNA content was determined by flow cytometry.
Results:Results revealed that the levels of HIF-1α protein increased in SSCs with the DMOG treatment in a dose-dependent manner. The relative transcription levels of SSCspecific genes were significantly upregulated (p < 0.05) by activating HIF-1α expression. The induction results showed that DMOG significantly increased (p < 0.05) the spermatogenesis capability of SSCs, and the populations of haploid cells significantly increased (p < 0.05) in DMOG-treated SSCs when compared to those in DMOG-untreated SSCs.
Conclusion:We demonstrate that DMOG can promote the spermatogenesis activity of SSCs.
