Effects of Lobetyolin, Lobetyol and Methyl linoleate on Secretion, Production and Gene Expression of MUC5AC Mucin from Airway Epithelial Cells.
10.4046/trd.2014.77.5.203
- Author:
Yong Pill YOON
1
;
Jiho RYU
;
Su Hyun PARK
;
Hyun Jae LEE
;
Seungho LEE
;
Sang Kook LEE
;
Ju Ock KIM
;
Jang Hee HONG
;
Jeong Ho SEOK
;
Choong Jae LEE
Author Information
1. Department of Pharmacology, Chungnam National University School of Medicine, Daejeon, Korea. LCJ123@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Mucins;
MUC5AC Protein, Human;
Methyl Linoleate
- MeSH:
Codonopsis;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells*;
Gene Expression*;
Linoleic Acid*;
Mucins*;
Polymerase Chain Reaction;
Reverse Transcription
- From:Tuberculosis and Respiratory Diseases
2014;77(5):203-208
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: In this study, we investigated whether lobetyolin, lobetyol, and methyl linoleate derived from Codonopsis pilosula affect MUC5AC mucin secretion, production, and gene expression from airway epithelial cells. METHODS: Confluent NCI-H292 cells were pretreated with lobetyolin, lobetyol, or methyl linoleate for 30 minutes and then stimulated with phorbol 12-myristate 13-acetate (PMA) for 24 hours. The MUC5AC mucin gene expression, and mucin protein production and secretion were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. RESULTS: Lobetyolin, lobetyol, and methyl linoleate inhibited the gene expression of MUC5AC mucin induced by PMA; lobetyolin did not affect PMA-induced MUC5AC mucin production. However, lobetyol and methyl linoleate inhibited the production of MUC5AC mucin; lobetyolin and lobetyol did not significantly affect PMA-induced MUC5AC mucin secretion from NCI-H292 cells. However, methyl linoleate decreased the MUC5AC mucin secretion. CONCLUSION: These results suggest that among the three compounds, methyl linoleate can regulate gene expression, production, and secretion of MUC5AC mucin by directly acting on the airway epithelial cells.
