Comparative Analysis of Cutaneous Fungi in Atopic Dermatitis Patients and Healthy Individuals
- Author:
Yoojeong CHOI
1
;
Kui Young PARK
;
Hye Sung HAN
;
Mi-Kyung LEE
;
Seong Jun SEO
Author Information
- Publication Type:Original Article
- From:Annals of Dermatology 2022;34(2):118-124
- CountryRepublic of Korea
- Language:English
-
Abstract:
Background:Atopic dermatitis (AD) is a chronic relapsing inflammatory skin disease triggered by diverse factors. Microbes are one of the crucial risk factors for AD development or exacerbation. However, the effect of a fungal burden on AD has been overlooked compared to bacteria.
Objective:This study aimed to comparatively analyze cutaneous fungal distribution between AD patients and healthy individuals by polymerase chain reaction (PCR)-based analysis.
Methods:Skin samples of AD outpatients and healthy individuals collected at the ChungAng University were analyzed. Representative AD-associated fungal genera, Candida, dermatophytes, and Malassezia, were analyzed using specific primer and amplification methods. Amplicons were sequenced, and the fungal distribution of both groups were compared.
Results:Totally, 211 patients and 23 healthy individuals were studied. Of the 211 patients, 10.90% (23/211) had Candida species, whereas 0% (0/23) healthy individuals showed its presence. The most frequently detected species in patients was Candida albicans (5.21%) followed by Candida parapsilosis (3.79%). For dermatophytes, 1.42% (3/211) of patients showed positive results, whereas 0% (0/23) healthy individuals showed positive results. Malassezia species were identified in 20.85% (44/211) and 8.70% (2/23) in patients and healthy individuals, respectively. Malassezia restricta was the most frequently identified species in the AD patient group, and the only species found in the healthy control group.
Conclusion:The distribution of Candida spp., dermatophytes, and Malassezia spp. are altered with AD development.
