Study on Q-markers of Da Chaihu decoction based on specific chromatogram and network pharmacology
10.16438/j.0513-4870.2021-1800
- VernacularTitle:基于特征图谱及网络药理学的大柴胡汤质量标志物研究
- Author:
Tian-yi LÜ
1
;
Ya-nan LIU
1
;
Yue REN
1
;
Yu-bin XU
2
;
Yuan ZHANG
3
;
Sheng-li WEI
3
;
Yan-ling ZHANG
1
Author Information
1. Key Laboratory of TCM Information Engineering, National Administration of Traditional Chinese Medicine, School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 102488, China
2. Hebei Jujing Pharmaceutical Co., Ltd., Anguo 071000, China
3. Engineering Research Center of Standardized Production of Chinese Medicinal Materials, Ministry of Education, Beijing 102488, China
- Publication Type:Research Article
- Keywords:
Da Chaihu decoction;
quality marker;
virtual screening;
specific chromatogram;
network pharmacology
- From:
Acta Pharmaceutica Sinica
2022;57(5):1477-1485
- CountryChina
- Language:Chinese
-
Abstract:
Da Chaihu decoction is a classic prescription for the treatment of cholecystitis that is widely used in clinical practice, and has a definite curative effect. However, due to its diverse components and complex functions, the traditional indexes fail to capture its overall efficacy. Therefore, this study analyzed and predicted the quality markers (Q-markers) of Da Chaihu decoction based on specific chromatogram and network pharmacology to provide a reference for the comprehensive control of the quality. The study obtained 35 potential practical components of Da Chaihu decoction through virtual screening. The specific chromatogram of 15 batches of Da Chaihu decoction was established by HPLC-DAD with neohesperidin as a reference. Compared with the chromatographic peaks and the reference substance, the chemical components were assigned to predict the nine components of albiflorin, paeoniflorin, naringin, hesperidin, neohesperidin, baicalin, wogonoside, saikosaponin b2, saikosaponin b1 as Q-markers of Da Chaihu decoction. Finally, the network of the "components-key targets-signal pathways-biological processes" was constructed by network pharmacology to explore the mechanism of Da Chaihu decoction in treating cholecystitis to clarify the accuracy of Q-markers. The results indicated that potential Q-markers could act on multiple targets to regulate inflammatory and metabolism, and then combine to treat cholecystitis. Q-markers could combine with the pharmacologic action of Da Chaihu decoction, which could elucidate the overall efficacy of Da Chaihu decoction. This study explored the Q-markers of Da Chaihu decoction combined with the specific chromatogram and network pharmacology, which provided a basis for the quality control and evaluation of Da Chaihu decoction.
