Cloning and subcellular location of the PnMYB1R1 gene from Panax notoginseng and expression analysis with different stresses
10.16438/j.0513-4870.2021-1617
- VernacularTitle:三七PnMYB1R1基因的克隆、亚细胞定位与不同胁迫下的表达分析
- Author:
Yue SHI
1
;
Jin-hui MAN
1
;
Wei-ye JIANG
1
;
Jing-han ZHANG
1
;
Zhi-fei ZHANG
1
;
Guang-yao YIN
1
;
Xin WANG
1
;
Yu-ying HUANG
1
;
Yuan ZHANG
1
,
2
;
Xiao-hui WANG
1
,
3
;
Sheng-li WEI
1
,
2
Author Information
1. School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
2. Engineering Research Center of Good Agricultural Practice for Chinese Crude Drugs of Ministry of Education, Beijing 100102, China
3. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Research Article
- Keywords:
italic>Panax notoginseng;
italic>PnMYB1R1;
prokaryotic expression;
subcellular localization;
transcription activity;
expression analysis
- From:
Acta Pharmaceutica Sinica
2022;57(5):1506-1515
- CountryChina
- Language:Chinese
-
Abstract:
MYB transcription factors, one of the largest transcription factor families in plants, play an important role in signal transduction, plant growth and plant resistance. In this study a full-length cDNA of the PnMYB1R1 gene was cloned from Panax notoginseng. Sequence analysis, prokaryotic expression and purification, subcellular location, transcriptional activity analysis, tissue-specific analysis and expression analysis under different abiotic stresses was performed. The open reading frame (ORF) of PnMYB1R gene was 738 bp, encoding a protein of 245 amino acids with a predicted molecular mass (MW) of 27.0 kD. The sequence analysis and polygenetic analysis indicated that the PnMYB1R1 protein contains a conserved R3 domain, belonging to TRF-like protein in 1R-MYB-type transcription factors. The recombinant PnMYB1R1 protein was expressed in Escherichia coli BL21(DE3) cells using the prokaryotic expression vector pET28a-PnMYB1R1 and was purified. Subcellular localization analysis showed that PnMYB1R1 was localized in the nucleus. Transcriptional activity analysis indicated that the PnMYB1R1 transcription factor has transcriptional activation activity. Expression analysis indicated that PnMYB1R1 was primarily expressed in roots, followed by stems and leaves, and then rootlets. The expression level of PnMYB1R1 in root, stems, leaves and rootlets was influenced by salt, low temperature and drought treatment, while the abundance of PnMYB1R1 was significantly induced by salt stress in these tissues. These results provide valuable insights into the role of 1R-MYB transcription factors in plant defense.
