Effect of Electroacupuncture with Low Frequency on Pain Sensitization of Rats Suffered from Spinal Nerve Ligation
10.3969/j.issn.1006-9771.2014.03.003
- VernacularTitle:低频电针对脊神经结扎大鼠痛敏化的干预作用
- Author:
Yongliang JIANG
1
;
Xiaohu YIN
1
;
Yafang SHEN
1
;
Xiaoyun ZHAO
1
;
Xiaofen HE
1
;
Jianqiao FANG
1
Author Information
1. Department of Neurobiology and Acupuncture Research, the Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, Zhejiang, China
- Publication Type:Journal Article
- Keywords:
neuropathic pain, electroacupuncture, dorsal root ganglion, transient receptor potential vanilloid type l, substance P
- From:
Chinese Journal of Rehabilitation Theory and Practice
2014;(3):211-214
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electroacupuncture with low frequency on pain sensitization of neuropathic pain rats,and the possible mechanism of regulating peripheral pain sensitization. Methods Rat neuropathic pain model was established with the right L5 spinal nerve ligation. Zusanli (ST36) and Kunlun (BL60) were selected for electroacupuncture intervention. Mechanical allodynia, and the levels of stransient receptor potential vanilloid type 1 (TRPV1) and substance P (SP) in ipsilateral L5 and L4 dorsal root ganglion (DRG) were tested. A further validation experiment with TRPV1 agonist 6'-IRTX was carried out. Results Rats suffered from L5 spinal nerve ligation showed obvious pain sensitization. The levels of TRPV1 in ipsilateral L4 DRG and SP in ipsilateral L5 DRG were significantly elevated.Electroacupuncture with low frequency significantly relieved pain sensitization, and suppressed TRPV1 and SP increase. 6'-IRTX intraperitoneally administered significantly counteracted the anti-pain sensitization action of electroacupuncture with low frequency. Conclusion Electroacupuncture with low frequency is effective on neuropathic pain by intervening pain sensitization, which may be related to the regulation
of DRG TRPV1 and SP.
