Anti-influenza Virus Activity in Agricultural, Forest and Fishery Products from Ishikawa Prefecture
- VernacularTitle:石川県産農林水産物の抗インフルエンザウイルス作用
- Author:
Emiko NAGAI
1
;
Mizuho OKUDA
1
;
Nobutaka SUZUKI
2
;
Masahiko TAKINO
3
;
Yuji ISEGAWA
4
;
Toshiki ENOMOTO
1
Author Information
- Keywords: Agricultural, Forest and Fishery Products; Kaga vegetables; Anti-influenza activity
- From:Japanese Journal of Complementary and Alternative Medicine 2022;19(1):23-31
- CountryJapan
- Language:Japanese
- Abstract: Objective: A study was conducted to clarify the anti-influenza virus effect of agricultural, forest and fishery products in Ishikawa Prefecture. Method: The samples were prepared by both hot-water and ethanol extraction. The Madin-Darby canine kidney (MDCK) cells infected with PR8 strain were cultured in Dulbecco's Modified Eagle Medium（DMEM） containing extract for 24 hours and measured virus titer of supernatants by focus-forming reduction assay. Time-of-addition assay was performed to determine the inhibition stage in virus proliferation by the extract. The active ingredients were purified using a solid-phase extraction column (silica gel base C18). Results: We revealed that many products have anti-influenza virus activity. Especially, four products ( Matteuccia struthiopteris, Colocasia esculenta, Sagittaria trifolia, and Eleucine coracana) show strong activity and inhibit several stages in virus proliferation. Conclusion: We performed screening to 46 agricultural, forest and fishery products and found the four products ( Matteuccia struthiopteris, Colocasia esculenta, Sagittaria trifolia, and Eleusine coracana) inhibit virus proliferation strongly. In addition, the isolation and identification of the active ingredients contained in these remain as a future study.
