Rapid analysis and identification of chemical components of ethanol extract from Mongolian medicine Sanzi san by HPLC-Q-Exactive-MS
- VernacularTitle:蒙药三子散醇提物化学成分的HPLC-Q-Exactive-MS快速分析与鉴定
- Author:
Jun LI
1
,
2
,
3
;
Yuxia HU
1
,
2
,
3
;
Mengdi ZHANG
3
;
Yuewu WANG
1
,
2
,
3
;
Qian ZHANG
1
,
2
,
3
;
Gejihu HU
4
;
Feng GAO
3
;
Fuhou CHANG
3
Author Information
1. The Center for New Drug Safety Evaluation and Research，Inner Mongolia Medical University，Hohhot 010110，China
2. Engineering Research Center of New Pharmaceutical Screening，Inner Mongolia Medical University，Hohhot 010110，China
3. College of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China
4. Dept. of Anesthesiology，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010110，China
- Publication Type:Journal Article
- Keywords:
Mongolian medicine;
Sanzi san;
HPLC-Q-
- From:
China Pharmacy
2022;33(11):1348-1354
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To systematic ally stu dy the chemic al components of ethanol extract from Sanzi san ，and to provide reference for clarifying the pharmacodynamic material basis of the formulation. METHODS HPLC-Q-Exactive-MS technology was adopted. The determination was performed on Shim-pack GIST-HP C 18 column with mobile phase consisted of acetonitrile- 0.1％ formic acid aqueous solution for gradient elution at the flow rate of 1 mL/min. The column temperature was 40 ℃，and sample size was 10 μL. Mass spectrometry conditions included the electrospray spray ion source was used for detection in positive and negative ion detection modes. Full MS/dd-MS 2 detection mode was adopted ，the resolution of Full MS was 70 000 and the resolution of dd-MS2 was 17 500. The scanning range was m/z 110-1 200. The ion peaks were identified by comparing with the information of control substances ，literature references and self-built database. RESULTS A total of 64 components were identified in the ethanol extract of Mongolian medicine Sanzi san ， including 9 flavonoids，13 iridoids，14 organic acids ，18 tannins，3 triterpenes，3 amino acids and 4 fatty acids. CONCLUSIONS The ethanol extract of Mongolian medicine Sanzi san mainly include iridoids ，tannins and flavonoids ，which might be the pharmacodynamic material basis of Sanzi san.
