Screening of active components of Euchresta japonica against nasopharyngeal carcinoma
- VernacularTitle:山豆根抗鼻咽癌活性成分的筛选
- Author:
Junling NAN
1
;
Chaozhong WANG
1
;
Guangli YAN
1
;
Fangfang WU
2
;
Zhijun SONG
2
;
Shuo WANG
2
;
Xiaolei ZHOU
2
;
Jianhua MIAO
2
;
Xijun WANG
1
Author Information
1. Chinmedomics Research Center of National Administration of Traditional Chinese Medicine/Research Center for Effectiveness Evaluation and Industrialization Development Engineering of Classical Formula of the Ministry of Education，Heilongjiang University of Chinese Medicine，Harbin 150040，China
2. Guangxi Botanical Garden of Medical Plants/Southwest National Engineering Laboratory for the Resources Development of Endangered Medic inal Mate rials，Nanning 530023，China
- Publication Type:Journal Article
- Keywords:
Euchresta japonica;
nasopharyngeal carcinoma;
active components;
tonkinensisol;
sophoranone;
maackiain
- From:
China Pharmacy
2022;33(11):1320-1329
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To screen t he active component s of Euchresta japonica against nasopharyngeal carcinoma. METHODS Main chemical components of E. japonica were selected ，and their target proteins were predicted in Swiss Target Prediction database. The target proteins of nasopharyngeal cancer were obtained with GeneCards database. Protein-protein interaction（PPI）network was established after the target of chemical components of E. japonica was intersected with the target of nasopharyngeal carcinoma ；PPI network was analyzed by using Cytoscape 3.6.1 software，and the potential active components and key targets of E. japonica against nasopharyngeal carcinoma were screened. The molecular docking technology was used to evaluate binding ability of active component-key target ；active components of E. japonica against nasopharyngeal carcinoma were screened. The anti-nasopharyngeal cancer effect of potential active components of E. japonica was verified by cell proliferation experiment. RESULTS Seven potential active components （tonkinensisol，quercetin，sophoranone，matrine，genistein，coumarin，maackiain） and 10 core targets （SRC，PIK3CA，MAPK1，MAPK3，AKT1，MAPK8，MAP2K1，PTK2，EGFR，JAK3）of E. japonica against nasopharyngeal carcinoma were screened. The molecular docking results showed that above potential active components all possessed certain anti-nasopharyngeal cancer effect. Cell proliferation activity test showed that tonkinensisol ，sophoranone and maackiain had a very significant inhibitory activity on nasopharyngeal carcinoma cells CNE- 1. CONCLUSIONS Tonkinensisol， sophoranone and maackiain might be the main active components of E. japonica against nasopharyngeal carcinoma.
