Study on regulatory mechanism of compatibility of ginseng and gecko dispensing granule on neuroendocrine immune network in model rats with kidney yang deficiency
- VernacularTitle:人参与蛤蚧配方颗粒配伍对肾阳虚模型大鼠神经内分泌免疫网络的调节机制研究
- Author:
Bei ZHOU
1
;
Qiaofeng ZHU
1
;
Yudan CHEN
2
;
Liting FENG
3
;
Shuling LIU
1
;
Yanchun WU
1
Author Information
1. College of Pharmacy，Guangxi University of Chinese Medicine，Nanning 530200，China
2. Dept. of Pharmacy，Guangxi Agricultural Vocational College，Nanning 530007，China
3. Dept. of Pharmacy，Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine，Nanning 530012，China
- Publication Type:Journal Article
- Keywords:
ginseng;
gecko;
compatibility;
kidney yang deficiency;
hypothalamus-pituitary-adrenal axis;
hypothalamus-
- From:
China Pharmacy
2022;33(11):1313-1319
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To explore the regulatory mechanism of compatibility of ginseng and gecko dispensing granule on kidney yang deficiency model rats. METHODS Male SD rats were randomly divided into normal group （no modeling ，no administration），model group （modeling，no administration ），Jinkui shenqi pill group （modeling，dose of 2.33 g/kg），ginseng group（modeling，dose of 0.53 g/kg），gecko group （modeling，dose of 0.21 g/kg）and compatibility group （modeling，ginseng 0.53 g/kg and gecko 0.21 g/kg）. The body mass and anal temperature of rats were measured at different time points ；the serum levels of cAMP ，cGMP，CRH，ACTH，CORT，T，T3，T4，E2，IgG and IgM were measured ；the pathomorphological changes of adrenal gland ，thyroid gland and testis were observed ；mRNA expression of CRH ，thyroid stimulating hormone releasing hormone （TRH）and gonadotropin releasing hormone （GnRH）in hypothalamus were detected. RESULTS Compared with model group ，the anal temperature ，the levels of cAMP ，CRH，ACTH，CORT，T3，T and cAMP/cGMP ，T/E2 in serum and mRNA expressions of TRH and GnRH in hypothalamus were significantly increased in the compatibility group （P＜0.05 or P＜0.01）；the levels of cGMP，E2 and IgG in serum and mRNA expression of CRH in hypothalamus decreased significantly （P＜0.05 or P＜0.01）； the pathological injuries of adrenal gland ，thyroid gland and testis were all improved. Compared with ginseng or gecko dispensing granules alone ，the anal temperature and T/E 2 of rats in the compatibility group increased significantly ，and mRNA expression of CRH in hypothalamus decreasedsignificantly （P＜0.05 or P＜0.01）. CONCLUSIONS Thecompatibility of ginseng and gecko dispensing granule has a synergistic regulatory effect on kidney yang deficiency model rats ， the mechanism of which may be associated with hypothalamus-pituitary-adrenal axis ， hypothalamus-pituitary-thyroid axis ， hypothalamus-pituitary-gonad axis and neuroendocrine immune network formed by immune function. Compatible drugs are better than single drugs.
