Induction of vitreolysis and vitreous detachment with hyaluronidase and perfluoropropane gas.
- Author:
Se Woong KANG
1
;
Sung Min HYUNG
;
Mi Young CHOI
;
Jaeheung LEE
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords: vitreous detachment; hyaluronidase; perfluoropropane gas
- MeSH: Animals; Drug Combinations; Electroretinography; Eye Diseases/chemically induced/pathology; Fluorocarbons/*toxicity; Hyaluronoglucosaminidase/*toxicity; Injections; Rabbits; Retina/drug effects/physiology; Vitreous Body/*drug effects/pathology
- From:Korean Journal of Ophthalmology 1995;9(2):69-78
- CountryRepublic of Korea
- Language:English
- Abstract: To simulate the posterior vitreous detachment (PVD) in the rabbit, 1 IU hyaluronidase and/or 0.2 ml of perfluoropropane gas was intravitreally injected. Ophthalmoscopic, light microscopic examination prepared by cryotechnique, electron microscopic examination, and electroretinogram were done on the 3rd and 28th postoperative days. As a result, the eyes undergone simultaneous intravitreal injection of 1 IU hyaluronidase and 0.2 ml perfluoropropane gas showed membranous structure split from the internal limiting membrane of the superior retina in 3 days after injection. The eyes also demonstrated membranous structure separated from the superior retina after 28 days, simulating vitreous detachment. On the contrary, neither agent alone induced vitreous detachment. No toxic retinal changes associated with simultaneous intravitreal injection of 1 IU hyaluronidase and 0.2 ml perfluoropropane gas were observed. Therefore, with a future support by histologic examination other than cryotechnique and by immunohistochemical analysis, the simultaneous intravitreal injection of perfluoropropane gas and hyaluronidase would be a promising method to induce vitreous detachment in non-vitrectomized eye.
