Effects of Electroacupuncture at Shenting and Baihui on Ability of Learning and Memory, and LIM-domain Containing Protein Kinase 1 Phosphorylation in Rats with Cerebral Ischemia-reperfusion
10.3969/j.issn.1006-9771.2016.11.002.
- VernacularTitle:电针神庭、百会对脑缺血再灌注大鼠学习记忆能力和LIM激酶1 磷酸化的影响①
- Author:
Jie WU
1
,
2
;
Peiyuan ZHUO
3
;
Yunjiao LIN
3
,
4
;
Lulu WANG
3
,
4
;
Jia HUANG
1
;
Ruhui LIN
5
;
Jing TAO
1
;
Weilin LIU
1
Author Information
1. 1. College of Rehabilitation Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
2. 2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350122, China
3. 2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350122, China
4. 3. Rehabilitation of Fujian Province Industry Research Institute, Fuzhou, Fujian 350122, China
5. 3. Rehabilitation of Fujian Province Industry Research Institute, Fuzhou, Fujian 350122, China
- Publication Type:Journal Article
- Keywords:
cerebral ischemia-reperfusion, electroacupuncture, learning and memory, synaptic plasticity, LIM-domain containing protein kinase 1, phosphorylation, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2016;22(11):1246-1247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of electroacupuncture (EA) at Shenting (DU24) and Baihui (DU20) acupoints on hippocampal synaptic plasticity and learning and memory function, and its possible mechanism in rats with cerebral ischemia-reperfusion. Methods Thirty-two male adult Sprague-Dawley rats were randomly divided into sham group (n=8), model group (n=8), electroacupuncture (EA) group (n=8) and non-acupoint group (n=8). The model group, EA group and non-acupoint group were performed with left middle cerebral artery occlusion (MCAO). The EA group received electroacupuncture at Shenting and Baihui, while the non-acupoint group received electroacupuncture at two fixed non-acupoints from bilateral ribs, for fourteen days. Then they were tested with Morris Water Maze test, and their synaptic structure of hippocampal neurons was observed with transmission electron microscope. The level of total LIM-domain containing protein kinase 1 (T-LIMK1) and LIM-domain containing protein kinase 1 phosphorylation (p-LIMK1) were detected with Western blotting. Results Compared with the sham group, the latency increased (t>6.789, P<0.01) and the frequence crossing platform decreased (t= 8.695, P<0.001) in the model group, while the number of synapse in hippocampal neurons decreased, and the level of T-LIMK1 (t=7.568, P< 0.01) and p-LIMK1 (t=8.874, P<0.001) decreased. Compared with the model group, the latency decreased (t>4.938, P<0.01) and the frequence crossing platform increased (t=- 7.891, P<0.001) in the EA group, while the number of synapses increased, and the level of T-LIMK1 (t=-6.473, P<0.01) and p-LIMK1 (t=-6.579, P<0.01) increased. There was no significant difference between the the non-acupoint group and the model group in all the indices (P>0.05). Conclusion Electroacupuncture at Shenting and Baihui can improve the ability of learning and memory in rats with cerebral ischemia-reperfusion, which may relate with the increase of LIMK1 phosphorylation and hippo-campal synaptic plasticity.
