Screening of effective anti-depressant part from Coreopsis tinctoria and study on its mechanism
- VernacularTitle:雪菊抗抑郁有效部位的筛选及其作用机制研究
- Author:
Rui MA
1
;
Shuang LI
1
;
Zhen WANG
1
;
Juanjuan GAO
1
;
Aidi XIE
1
;
Haixia HAN
1
Author Information
1. College of Food Science and Pharmacy，Xinjiang Agricultural University，Urumqi 800052，China
- Publication Type:Journal Article
- Keywords:
Coreopsis tinctoria;
anti-depressant;
effective part;
monoamine neurotransmitter;
5-hydroxytryptamine
- From:
China Pharmacy
2022;33(10):1177-1182
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To screen the effective anti-depressant part from Coreopsis tinctoria and study its mechanism. METHODS The anti-depressant effects of 30％，50％，70％ and 90％ ethanol elution fractions from 75％ ethanol extract of C. tinctoria（CCTE）were investigated by tail suspension test and forced swimming test. Mice head-drop test ，reserpine antagonistic test，yohimbine toxicity enhancement test and in vitro monoamine oxidase （MAO） inhibition test were used to explore the mechanism of the relationship between the effective parts and 5-hydroxytryptamine （5-HT） and norepinephrine （NE） nerves. RESULTS The 50％ and 70％CCTE could significantly shorten the accumulative immobility time in tail suspension test and forced swimming test （P＜0.05 or P＜0.01），increase the number of head-shaking times （P＜0.01），reverse the eyelid ptosis ， hypothermia and immobility caused by hematopin （P＜0.05 or P＜0.01），and increase the number of dead mice caused by yohimbine toxicity （P＜0.01）. IC 50 of okanin （CCT-6），isookanin（CCT-7）and taxifolin （CCT-8）against MAO were 8.71，37.89 and 67.07 µmol/L，respectively. CONCLUSIONS The 50％ and 70％CCTE are the effective anti-depressant parts of C. tinctoria . Its anti-depressant effect may be related to the reinforcement of 5-HT and the activation of NE nerves. The inhibition of CCT- 6， CCT-7 and CCT- 8 against MAO may be one of the anti-depressant mechanism of C. tinctoria .
