Determination of total polyphenols and catechins in betel nut polyphenol extract
10.12206/j.issn.1006-0111.202106013
- VernacularTitle:槟榔多酚提取物中总多酚和儿茶素类的含量测定
- Author:
Jianghong MA
1
,
2
;
Rong WANG
2
;
Xing DU
3
;
Anpeng ZHAO
2
;
Zihan WANG
1
,
2
;
Haobo ZHANG
4
Author Information
1. School of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China
2. Deperment of pharmacy, No. 940 Hospital of Joint Logistics Support Force of the PLA, Lanzhou 730050, China.
3. Gansu Institute for Drug Control, Lanzhou 730070, China.
4. School of Pharmacy, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, China.
- Keywords:
betelnut total polyphenols;
catechins;
HPLC;
UV spectrophotometry
- From:
Journal of Pharmaceutical Practice
2022;40(3):243-247
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of total polyphenols and catechins in betel nut polyphenols extract, and provide reference for the quality control of betel nut polyphenols extract. Methods The content of total phenol in betel nut extract was determined by ultraviolet spectrophotometry. The content of catechins was determined by HPLC. Results The linear range of total polyphenols in betel nuts extract was 9.8~58.8 μg/ml. The three components of catechin, epicatechin and protocatechuic acid were completely separated by HPLC, and the linear relationship was good in their respective ranges, with the recoveries between 99.17% and 101.67%, the RSD between 1.2% and 2.5%. Conclusion The established method is simple, stable and reliable, which could be used for the quantitative analysis of betel polyphenol extract, and provide experimental basis for the quality control of betel polyphenol extract.
