Analysis of chemical ingredients of modified Shenqi Dihuang decoction by UPLC-LTQ-Orbitrap-MS
10.12206/j.issn.1006-0111.202112054
- VernacularTitle:基于UPLC-LTQ-Orbitrap-MS分析加味参芪地黄汤的化学成分
- Author:
Zhong WAN
1
;
Wangzhenzu LIU
2
;
Mingyue TAN
1
;
Dongliang XU
1
;
Cheng HU
2
;
Wanli JI
3
Author Information
1. Urinary Center, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
2. .
3. School of Pharmacy, Shanghai University of Medicine & Health Sciences, Shanghai 201318, China.
- Keywords:
Shenqi Dihuang decoction;
chemical constituents;
ultra performance liquid chromatography-ion trap-electrostatic field orbitrap mass spectrometry
- From:
Journal of Pharmaceutical Practice
2022;40(3):231-237
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the chemical compounds of Shenqi Dihuang decoction by the ultraperformance liquid chromatography coupled with linear quadrupole ion trap-orbitrap mass spectrometry (UPLC-LTQ-Orbitrap-MS). Methods Warters ACQUITY UPLC HSS T3 (2.1 mm ×100 mm, 1.8 μm) was used as chromatographic column with mobile phase: 0.1% formic acid water (A)-0.1% formic acid acetonitrile (B) with gradient elution, and flow rate was 0.3 ml/min. Electrospray ion source (ESI) and an electrostatic field orbital ion trap mass analyzer were adopted, which was used to collect mass spectrometry fragment information with positive and negative ion modes, by comparing with the relative retention time of the reference substance. In addition, the fragment information of the mass spectrum was used to identify the compounds. The accurate identification of the chemical components in Shenqi Dihuang decoction was confirmed with literature. Results The study found that UPLC-LTQ-Orbitrap-MS technology could be used to identify 62 chemical components, including 13 aromatic acids, 9 flavonoids, 8 saponins, and 5 aromatic amines, 3 keto acids, 2 phenols, 1 aromatic quinone and other ingredients in Shenqi Dihuang decoction. Conclusion The identification analysis method in this study was efficient and accurate, which could be applied to the identification and analysis of chemical components in Shenqi Dihuang decoction and provided the important experimental data for the research on the material basis and mechanism.
