Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)

Wenhao Zhang; Jianjun LI; Degang Yang; Mingliang Yang; Liangjie DU; Feng Gao; Changbin Liu; Dapeng LI; Anming HU; Chang Cai

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Research Progress of Two-photon Microscopy in Small Animals in Vivo Imaging (review)

VernacularTitle:双光子显微镜在小动物活体光学成像中的研究进展
Author: Wenhao ZHANG ^{1
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5} ; Jianjun LI ^{1
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5} ; Degang YANG ^{1
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5} ; Mingliang YANG ^{1
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5} ; Liangjie DU ^{1
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5} ; Feng GAO ^{1
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5} ; Changbin LIU ^{1
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5} ; Dapeng LI ^{1
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5} ; Anming HU ¹ ; Chang CAI ^{1
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Author Information

1. 1. Capital Medical University School of Rehabilitation Medicine, Beijing 100068, China
2. 2. a. Department of Spinal and Neural Function Reconstruction,China Rehabilitation Research Center, Beijing Bo'
3. ai Hospital, Beijing 100068, China
4. 3. Center of Neural Injury and Repair, Beijing Institute for Brain Disorders, Beijing 100068, China
5. 4. Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing 100068, China
6. 4. Beijing Key Laboratory of Neural Injury and Rehabilitation, Beijing 100068, Chinab. Department of Neurosurgery, China Rehabilitation Research Center, Beijing Bo'
Publication Type:Journal Article
Keywords: two-photon microscopy, in vivo optical imaging, small animals, review
From: Chinese Journal of Rehabilitation Theory and Practice 2017;23(1):37-41
CountryChina
Language:Chinese
Abstract: Twophoton microscopy is a new technique which combines laser scanning con-focal microscopy and two-photon excitation technique. Two-photon fluorescence microscopy has the advantages of little light damage, small bleaching area, strong penetrability, high resolution, high fluorescence collection efficiency, and high image contrast. It is suitable for dark field imaging and multi-labeled compound measurement, and has been widely used in small animals in vivo optical imaging, such as research for tumour, gene therapy, stem cells, drug development, spinal cord injury, etc.