Effects of Tuina of Three Handing-Three Points on Motor Function and Expression of NogoA and Its Receptor in Spinal Cord of Rats with Sciatic Nerve Injury
10.3969/j.issn.1006-9771.2018.12.009
- VernacularTitle:“三法三穴”推拿手法对坐骨神经损伤大鼠运动功能和脊髓NogoA及其受体表达的影响
- Author:
Yizhen LI
1
;
Chao
1
;
Tianyuan YU
1
;
Mengqian LU
1
;
Runpei MIAO
1
;
Taotao LV
1
Author Information
1. Beijing University of Chinese Medicine, Beijing 100029, China
- Publication Type:Journal Article
- Keywords:
sciatic nerve injury, Tuina, NogoA, receptor, nerve regeneration, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(12):1403-1407
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Tuina of Three Handing-Three Points on hindlimb motor function and expression of protein NogoA and its receptor NgR in the spinal cord of rats with sciatic nerve injury. Methods A total of 64 male Sprague-Dawley rats were randomly assigned to normal group (n = 16), sham group (n = 16), model group (n = 16) and Tuina group (n = 16). The model group and Tuina group prepared the sciatic nerve injury model with brace method. Tuina group received Tuina in methods of pressing, strumming and circular rubbing on Yinmen (BL37), Chengshan (BL57) and Yanglingquan (GB34) with Tuina manipulation emulator. The rats were assessed with oblique plate test seven days after operation and 20 days after Tuina. Then, the spinal cord tissues were extracted to detect the expression of protein of NogoA and NgR with Western blotting. Results The scores of the oblique plate test decreased in the model group and Tuina group seven days after operation compared with those in the normal group (P < 0.05), while the expression NgR increased (P < 0.05). The scores of the oblique plate test increased in Tuina group compared with those in the model group 20 days after Tuina (P < 0.05), while the expression of NgR decreased (P < 0.05), similar to the normal group (P > 0.05). Conclusion Tuina of Three Handing-Three Points can improve the hindlimb motor function in rats with sciatic nerve injury, which may be related to inhibition of the expression of NgR in the spinal cord after sciatic nerve injury.
