Analysis of Physiologically Active Substances in Rat Spinal Synaptosome
10.3969/j.issn.1006-9771.2018.12.008
- VernacularTitle:大鼠脊髓突触体中生理活性物质分析
- Author:
Zheng LIU
1
,
2
;
Wenhua YUAN
1
,
2
;
Shuxuan ZHAO
1
,
2
;
Wei SHEN
1
,
2
;
Xiaofei FENG
1
,
2
;
Haiyu ZHOU
1
Author Information
1. 1. Department of Orthopedics, Second Hospital of Lanzhou University, Lanzhou, Gansu 730030, China
2. 2. Key Laboratory of Bone and Joint Diseases of Gansu Province, Lanzhou, Gansu 730030, China
- Publication Type:Journal Article
- Keywords:
spinal cord, synaptosomes, amino acid neurotransmitters, adenosine triphosphate, calcium ion, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(12):1398-1402
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the physiologically active substances, named amino acid neurotransmitters, ATP and Ca2+ in rat spinal synaptosomes. Methods The spinal synaptosome was extracted by discontinuous Percoll density gradient centrifugation from a female Sprague-Dawley rat. The amino acid neurotransmitters were determined with high performance liquid chromatography, ATP content, Na+-K+-ATPase activity, Ca2+-Mg2+-ATPase activity and total ATPase activity with ultraviolet spectrophotometer, and Ca2+ concentration with fluorescence spectrophotometer. Results The amino acid neurotransmitters contained mainly aspartic acid, glycine, glutamic acid and r-aminobutyric acid. The concentration of ATP was about 414.7461 μmol/mg, total ATPase activity > Na+-K+-ATPase activity > Ca2+-Mg2+-ATPasese activity. The concentration of Ca2+ could be calculated from absorbancy directly. Conclusion The rat spinal synaptosome contains a variety of physiologically active substances, which can be accurately analyzed to explore their activities.
