Effect of Echinococcus granulosus hydatid cyst fluid protein on allergic rhinitis induced by ovalbumin in mice
10.16250/j.32.1374.2021276
- VernacularTitle:细粒棘球蚴囊液蛋白对卵清蛋白诱导的 小鼠变应性鼻炎效果
- Author:
Hong-yu GAO
1
,
2
,
3
;
Chen WAN
1
,
3
,
4
;
Fa-di SUN
5
;
Shu-ying WANG
1
,
2
;
Liang CHU
5
;
Yuan YUAN
1
,
6
;
Pu WANG
1
;
Xue-qin YU
1
;
Wei-yue LIU
1
;
Huai-fu DONG
7
;
Xiao-di YANG
1
,
4
Author Information
1. Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, Bengbu, Anhui 233000, China
2. Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, China
3. Co-first authors
4. Department of Microbiology and Parasitology, Bengbu Medical College, Bengbu, Anhui 233000, China
5. Department of General Surgery, The Second Affiliated Hospital of Bengbu Medical College, China
6. Department of Basic Medical Sciences, Bengbu Medical College, China
7. Department of Pediatrics, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, China
- Publication Type:Journal Article
- Keywords:
Echinococcus granulosus;
Hydatid cyst fluid protein;
Ovalbumin;
Allergic rhinitis;
Mouse
- From:
Chinese Journal of Schistosomiasis Control
2022;34(2):158-162
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of Echinococcus granulosus hydatid cyst fluid protein (HCFP) on ovalbumin (OVA)-induced allergic rhinitis (AR) in mice. Methods Twenty-four BALB/c mice at ages of 8 to 10 weeks, each weighing approximately 20 g, were randomly divided into four groups, including groups A (blank control group), B (blank intervention group), C (AR model group) and D (AR+HCFP intervention group), with 6 mice in each group. On days 0, 2, 4, 6, 8, 10 and 12, mice in groups A, B, C and D were injected with 200 μL sterile phosphate buffered saline (PBS), 200 μL sterile PBS containing 20 μg HCFP, 200 μL sterile PBS containing 50 μg OVA and 5 mg Al(OH)3 gel, and 200 μL sterile PBS containing 50 μg OVA, 5 mg Al(OH)3 gel and 20 μg HCFP, respectively. On days 14 to 20, mice in groups A, B, C and D were administered with 40 μL sterile PBS, 40 μL sterile PBS containing 20 μg HCFP, 40 μL sterile PBS containing 2 mg OVA and 40 μL sterile PBS containing 2 mg OVA and 20 μL HCFP by nasal drop, respectively. Mouse behavioral changes were observed and behavioral scores were estimated. The serum levels of interferon-γ (IFN-γ), interleukin-4 (IL-4), IL-5, IL-10, transforming growth factor-β (TGF-β) and OVA-specific IgE antibody (OVA-sIgE) were measured using enzyme-linked immunosorbent assay (ELISA), and the pathological changes of mouse nasal mucosa were observed by hematoxylin and eosin (HE) staining. Results The mean behavioral score was significantly greater in Group C (6.83 ± 0.50) than in groups A (1.17 ± 0.52) and B (1.33 ± 0.52) (P < 0.05), while a lower mean behavioral score was estimated in Group D (3.50 ± 0.50) than in Group C (P < 0.05). There were significant differences among the groups in terms of serum IFN-γ (F = 4.08, P < 0.05), IL-4 (F = 275.90, P < 0.05), IL-5 (F = 96.82, P < 0.05), IL-10 (F = 77.67, P < 0.05), TGF-β (F = 9.98, P < 0.05) and OVA-sIgE levels (F = 44.69, P < 0.05). The serum IFN-γ level was significantly lower in Group C than in groups A, B and C (P < 0.05), and the serum levels of IL-4, IL-5 and OVA-sIgE were significantly higher in Group C than in groups A, B and C (P < 0.05), while the serum IL-10 and TGF-β levels were significantly greater in Group D than in Group C (P < 0.05). Microscopy showed apparent loss of nasal mucosa cilia, increased number and enlargement of goblet cells, interstitial edema and submucous vascular dilation in Group C, while the pathological changes of nasal mucosa were alleviated in Group D relative to Group C. Conclusions E. granulosus HCFP has a protective activity against OVA-induced allergic rhinitis in mice.
