Effects of compatibility of Schisandra chinensis on Psoralea corylifolia -induced oxidative damage and endoplasmic reticulum stress in hepatocytes
- VernacularTitle:五味子配伍对补骨脂致肝细胞氧化损伤和内质网应激的影响
- Author:
Jingxi ZHANG
1
;
Jia YIN
2
;
Xiaolin QU
1
;
Hui LIU
1
;
Na WANG
1
;
Jiajing YAN
1
;
Chunsheng FU
3
Author Information
1. School of Pharmacy，Shandong University of Traditional Chinese Medicine，Jinan 250355，China
2. Dept. of Breast and Thyroid Surgery，the Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Jinan 250014，China
3. Dept. of Science and Technology，the Affiliated Hospital of Shandong University of Traditional Chinese Medicine，Jinan 250014，China
- Publication Type:Journal Article
- Keywords:
Schisandra chinensis;
Psoralea corylifolia;
compatibility attenuation;
liver injury;
oxidative damage;
endoplasmic
- From:
China Pharmacy
2022;33(9):1088-1093
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the effects of the compatibility of Schisandra chinensis on Psoralea corylifolia -induced oxidative damage and endoplasmic reticulum stress in L 02 hepatocytes，and to provide reference for clinical use of the compatibility attenuation of P. corylifolia -S. chinensis . METHODS L02 cells were divided into blank control group ，P. corylifolia model group （1 200 μg/mL P. corylifolia ，calculated by crude drug ），P. corylifolia -S. chinensis low-dose，medium-dose and high-dose groups （1 200 μg/mL P. corylifolia combined with 600，1 200，2 400 μg/mL S. chinensis ，respectively，calculated by crude drug ）. After the cells in each group were cultured in culture medium or drug solution for 48 hours，the levels of aspartate aminotransferase（AST）and alanine aminotransferase （ALT）were detected ；the levels of glutathione （GSH），superoxide dismutase （SOD）and malondialdehyde （MDA）in cell culture medium were detected ；reactive oxygen species （ROS）level and mitochondrial membrane potential in cells were detected ；mRNA and protein expressions of glucose-regulated protein 78（GRP-78）and protein kinase R-like endoplasmic reticulum kinase （PERK）were detected. RESULTS Compared with blank control group ，the levels of AST，ALT，MDA and ROS ，mRNA and protein expressions of GRP- 78 and PERK were increased significantly in P. corylifolia model group （P＜0.05 or P＜0.01）；while GSH and SOD levels ，mitochondrial membrane potential were decreased significantly （P＜0.05 or P＜0.01）. Compared with P. corylifolia model group ，above indexes of P. corylifolia -S. chinensis low-dose， medium-dose and high-dose groups were all improved significantly （P＜0.05 or P＜0.01）. CONCLUSIONS The compatibility of P. corylifolia -S. chinensis can alleviate P. corylifolia -induced oxidative damage and endoplasmic reticulum stress of L 02 cells.
