Improvement effects of Dianxianqing granule on blood-brain barrier injury in Alzheimer ’s disease model mice
- VernacularTitle:癫痫清颗粒改善AD模型小鼠血脑屏障损伤的作用研究
- Author:
Yue QI
1
;
Zhao LI
2
;
Xia HOU
1
;
Peichi HUANG
1
;
Dong JIA
3
;
Caiyu YANG
3
;
Xiaobo DONG
3
;
Xiaonan FANG
2
;
Cairong MING
3
Author Information
1. Dept. of Pharmaceutical Technology，Jiangsu Provincial Xuzhou Pharmaceutical Vocational College，Jiangsu Xuzhou 221116，China
2. Dept. of Pharmacology，College of Pharmacy，Shenyang Medical College，Shenyang 110031，China
3. Dept. of Pharmacology，the Second Affiliated Hospital of Liaoning University of TCM，Shenyang 110034，China
- Publication Type:Journal Article
- Keywords:
Dianxianqing granule;
Alzheimer’s disese;
blood-brain barrier;
NLRP3 inflammasome;
tight junction proteins
- From:
China Pharmacy
2022;33(9):1062-1067
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To study the impr ovement effects of Dianxianqing granule on blood-brain barrier （BBB）injury in Alzheimer’s disease （AD）model mice by regulating NLR family pyrin domain containing 3（NLRP3）inflammasome signaling pathway. METHODS Totally 125 mice were randomly divided into sham operation group （n＝25）and modeling group （n＝100） by body weight. AD model was induced by intracerebroventricular injection of β-amyloid 25-35 in model group. Sham operation group was given normal saline with same method. The 100 model mice were randomly divided into model group ，Donepezil hydrochloride tablets group （positive control 1，1.3 mg/kg，i.g.），MCC950 group [positive control 2（selective NLRP 3 inhibitor），10 mg/kg，i.p.] and Dianxianqing granule group （12.48 g/kg by crude drug ，i.g.）by body weight ，with 25 mice in each group. Second day after modeling ，administration groups were given relevant medicine ，once a day ，for consecutive 21 d. Sham operation group and model group were given intragastric administration of water and intraperitoneal injection of normal saline. At last administration，the learning and memory ability was determined by Y maze test ，and blood-brain barrier permeability was measured by Evans blue leakage assay. The expressions of NLRP 3，anti-ionized calcium-binding adapter molecule 1（IBA-1），nuclear factor kappa B （NF-κB）p65，p53 upregulated modulator of apoptosis （PUMA），occludin（ocln），zonula occluden- 1（ZO-1）and claudin-5 （cldn5） in cerebral tissue were determined. RESULTS Compared with model group ， spontaneous alternate response rate ，protein expressions of ocln ，cldn5 lnzyxyqy2003@163.com and ZO- 1 in cerebral tissue were increased significantly in administration groups （P＜0.05 or P＜0.01）；Evans blue E-mail：jiadg2003@126.com content and protein expressions of NLRP 3，IBA-1，PUMA and NF-κB p65 in cerebral tissue were decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS Dianxianqing granule can improve BBB injury of AD model m ice by inhibiting NLRP 3 inflammasome signaling pathway.
