Effects of Du Meridian Moxibustion on Ubiquitin Proteasome Pathway in Skeletal Muscle of Aging Rats
10.3969/j.issn.1006-9771.2018.09.005
- VernacularTitle:督脉灸对衰老模型大鼠骨骼肌泛素蛋白酶体途径的影响
- Author:
Jianping LIN
1
,
2
;
Xi WANG
1
;
Wulong LIU
3
,
4
;
Yimin CHEN
1
;
Shaoqing CHEN
3
,
4
;
Shizhong WANG
5
Author Information
1. 1. Affiliated Rehabilitation Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350003, China
2. 2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350003, China
3. 2. Fujian Key Laboratory of Rehabilitation Technology, Fuzhou, Fujian 350003, China
4. 3. Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China
5. 4. Fujian Medical University, Fuzhou, Fujian 350122, China
- Publication Type:Journal Article
- Keywords:
Du Meridian moxibustion, skeletal muscle, aging, ubiquitin, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(9):1018-1021
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of ubiquitin proteasome pathway in Du Meridian moxibustion for skeletal muscle aging. Methods A total of 30 Sprague-Dawley rats were randomly separated into control group (n=10), model group (n=10) and moxibustion group (n=10). The model group and the moxibustion group were subcutaneously injected D-galactose 125 mg/kg daily for six weeks, while the control group was injected the same volume of saline. Then the moxibustion group was cauterized on Du Meridian 20 minutes a day for 20 days. The expression of ubiquitin, muscle RING finger 1 (MuRF1) and muscle atrophy F-box (MAFbx) in erector spinae were tested with Western blotting. Results The expression of ubiquitin, MuRF1 and MAFbx increased in the model group compared with that of the control group (P<0.05), decreased in the moxibustion group compared with that of the model group (P<0.05).Conclusion The Du Meridian moxibustion may regulate ubiquitin proteasome pathway to inhibit the degradation of protein, and delay muscular atrophy.
