Role of Ski in Inflammatory Factor Release in Activated Astrocytes
10.3969/j.issn.1006-9771.2018.07.008
- VernacularTitle:Ski对脂多糖诱导大鼠星形胶质细胞炎症因子释放的影响
- Author:
Ming WANG
1
,
2
;
Xinle YANG
3
;
Tiege CHEN
1
,
2
;
Dangling ZHANG
1
,
2
;
Zhaoyang GONG
1
,
2
;
Gao XIANG
1
,
2
;
Kaixin LIU
1
,
2
;
Haihong ZHANG
1
,
2
Author Information
1. 1. Department of Orthopedics, the Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China
2. 2. Lanzhou University Key Laboratory of Orthopedics of Gansu Province, Lanzhou, Gansu 730030, China
3. . Department of Orthopedics, the Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu 730030, China
- Publication Type:Journal Article
- Keywords:
Ski, RNA interference, lipopolysaccharide, inflammation, astrocytes, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(7):802-806
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Ski on the secretion of inflammatory cytokines from activated astrocytes. Methods Astrocytes were obtained from cerebral cortex of a three-day old Sprague-Dawley rat and cultured in vitro. They were divided into blank group, control group and siRNA group. The Ski gene was silenced in siRNA group. The expression of Ski was tested with Western blotting and immunofluorescence 48 hours later. Then the astrocytes were stimulated with lipopolysaccharide for 24 hours. The secretion of tumor necrosis factor α (TNF-α) and interleukin-1β (IL-1β) in activated astrocytes was detected with ELISA. Results The expression of Ski protein reduced in the siRNA group (P<0.001), as well as the secretion of TNF-α and IL-1β (P<0.001). Conclusion Ski may play a role in inflammatory response of astrocyte.
