Effects of Ginsenoside Rb1 in Different Dosages on Spinal Cord Ischemia-reperfusion Injury and Expression of Survivin in Rats
10.3969/j.issn.1006-9771.2018.05.006
- VernacularTitle:不同剂量人参皂甙Rb1对大鼠脊髓缺血再灌注损伤及survivin的作用
- Author:
Bin CHENG
1
,
2
,
3
;
Huanjin SONG
1
,
2
,
3
;
Fengtao LI
1
,
2
,
3
;
Lei LIN
4
;
Jianli XUE
1
,
2
,
3
;
Hao WU
1
,
2
,
3
;
Jintao YE
1
,
2
,
3
Author Information
1. Department of Orthopedics, the 2nd Affiliated Hospital of Xi'
2. an Jiaotong University, Xi'
3. an, Shaanxi 710004, China
4. Department of Orthopedics, Hanzhong Central Hospital, Hanzhong, Shaanxi 723000, China
- Publication Type:Journal Article
- Keywords:
spinal cord injury, ischemia-reperfusion, ginsenoside Rg1, survivin, rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2018;24(5):520-525
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of different dosages of ginsenoside Rb1 preconditioning on spinal cord ischemia-reperfusion injury in rats, and the possible mechanism. Methods Sprague-Dawley rats were randomly divided into sham group (n=12), model group (n=12), and 10 mg/kg (D10, n=12), 20 mg/kg (D20, n=12), 40 mg/kg (D40, n=12) and 80 mg/kg (D80, n=12) drug groups. Spinal cord ischemia for ten minutes and reperfusion model was established, and the drug groups were injected ginsenoside Rb1 intraperitoneally in their dosages 30 minutes before modeling. They were assessed with BBB score 48 hours after reperfusion, and then were sacrificed for HE staining, TUNEL staining and immunohistochemistry staining of survivin.Results The BBB score was more in the drug groups than in the model group (P<0.05), and was the most in D40 and D80 groups. The expression of survivin was more in the drug groups than in the model group (P<0.05), and was the most in D40 and D80 groups. The apoptosis of neurons was less in the drug groups than in the model group (P<0.05), and was the least in D40 and D80 groups.Conclusion The ginsenoside Rb1 could promote the expression of survivin, inhibit apoptosis of neurons, to protect the neural function, in dose-dependent manner somehow.
