Establisment of quantitative analysis of multi-components by singer marker for content determination of 4 carotenoids in Lycium barbarum
- VernacularTitle:枸杞子中4种类胡萝卜素成分含量测定一测多评法的建立
- Author:
Huan CHEN
1
,
2
;
Ling MA
1
;
Xiaojuan SU
3
;
Yanting LI
3
;
Xia MA
1
;
Xueqin MA
3
Author Information
1. Ningxia Hui Autonomous Region Institute of Drug Control，Yinchuan 750002，China
2. Ningxia Key Laboratory of Drug Creation and Generic Drug Research，Yinchuan 750004，China
3. College of Pharmacy，Ningxia Medical University，Yinchuan 750004，China
- Publication Type:Journal Article
- Keywords:
Lycium barbarum;
HPLC;
QAMS;
zeaxanthin;
β-carotene;
β-cryptoxanthin palmitate;
zeaxanthin dipalmitate
- From:
China Pharmacy
2022;33(8):957-961
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To establish a method for simultaneous determination of zeaxanthin ，β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate in Lycium barbarum . METHODS L. barbarum was extracted with n-hexane-anhydrous ethanol-acetone-toluene（10∶6∶7∶7，V/V/V/V）by ultrasonic method. High performance liquid chromatography （HPLC）method was adopted. The determination was performed on YMC C 30 column with mobile phase A consisted of methanol-acetonitrile-water （81∶ 14 ∶ 5，V/V/V）and mobile phase B consisted of dichloromethane （gradient elution ）at the flow rate of 1.0 mL/min. The column temperature was set at 20 ℃. The detection wavelength was set at 450 nm，and sample size was 20 μL. Using zeaxanthin as control，the relative correction factors （RCFs）of β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate were calculated ， and then the content of each component was calculated according to RCFs and compared with the results of external standard method（ESM）. RESULTS The linear range of zeaxanthin ，β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate were 0.119 4-2.983 8，0.121 7-1.521 6，0.285 9-5.718 8，8.460 5-211.513 3 μg/mL（all R2＞0.999）. RSDs of precision ，repeatability and stability（16 h）tests were all less than 4％. The average recoveries were 103.34％，107.37％，105.64％，96.16％（RSD＜5％，n＝ 9）. The average RCFs of β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate were 1.109，1.390，1.158. The relative errors of the content determination results by quantitative analysis of multi-components by singer marker （QAMS）and ESM were within ±1％. CONCLUSIONS The established HPLC-QAMS method is accurate and stable ，which can be used for the content determination and quality control of 4 carotenoids in L. barbarum .
