Protective effect and mechanism of Artemisia argyi extract on vancomycin-induced acute kidney injury
10.11665/j.issn.1000-5048.20220215
- VernacularTitle:艾叶提取物对万古霉素诱导急性肾损伤的保护作用及其机制
- Author:
Da LI
;
Min LI
;
Wenzhi CAI
;
Wenyuan LIU
;
Jian XU
;
Baowei YANG
;
Feng FENG
- Publication Type:Journal Article
- Keywords:
Artemisia argyi extract;
acute kidney injury;
mechanism;
vancomycin
- From:
Journal of China Pharmaceutical University
2022;53(2):239-244
- CountryChina
- Language:Chinese
-
Abstract:
The goal of the study was to investigate the protective effect and mechanism of Artemisia argyi ethanol extract on chemotherapeutic vancomycin (VAN)-induced acute kidney injury (AKI).The acute kidney injury model of male ICR mice was induced by intraperitoneal injection (ip) of VAN.Thirty mice were divided into the blank group, model group, high dose group, middle dose group and low dose group, which were given medicine by gastric perfusion (ig).Serum levels of cystain C (Cys C), blood urea nitrogen (BUN) and serum creatinine (Scr) were measured, which could reflect renal function of mice.Serum oxidative stress and inflammation indices were also determined, including muscular dystrophy association (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), tumor necrosis factor-alpha (TNF-α), Interleukin-1β (IL-1β), and high-sensitive c-reactive protein (hs-CRP).In addition, hematoxylin-eosin staining (HE) was employed for measuring the damage of renal tissues and the content of apoptosis b-cell lymphoma-2 (Bcl-2), Bcl-2 assaciated X protein (Bax) and caspase-3 were measured too.All results showed that Artemisia argyi extract exhibits protective effect on chemotherapeutic VAN-induced AKI, whose mechanism could be related to the oxidative stress, inflammatory reaction and apoptosis.
